Laboratory of Bio-Control and Molecular Biology, Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi Arabia.
Research and Consulting Institute, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi Arabia.
Molecules. 2019 Nov 26;24(23):4304. doi: 10.3390/molecules24234304.
Disruption in chitin regulation by using chitin synthesis inhibitor (novaluron) was investigated to gain insights into the biological activity of chitinase in red palm weevils, an invasive pest of date palms in the Middle East. Impact of novaluron against ninth instar red palm weevil larvae was examined by dose-mortality response bioassays, nutritional indices, and expression patterns of genes characterized in this study. Laboratory bioassays revealed dose-dependent mortality response of ninth-instar red palm weevil larvae with LD of 14.77 ppm of novaluron. Dietary growth analysis performed using different doses of novaluron (30, 25, 20, 15, 10, and 5 ppm) exhibited very high reduction in their indexes such as Efficacy of Conversion of Digested Food (82.38%) and Efficacy of Conversion of Ingested Food (74.27%), compared with control treatment. Transcriptomic analysis of red palm weevil larvae characterized numerous genes involved in chitin degradation including , , , , , . However, quantitative expression patterns of these genes in response to novaluron-fed larvae revealed tissue-specific time-dependent expression patterns. We recorded overexpression of all genes from mid-gut tissues. Growth retarding, chitin remodeling and larvicidal potential suggest novaluron as a promising alternate for management.
采用几丁质合成抑制剂（novaluron）干扰几丁质调控，研究了几丁质酶在入侵中东枣椰树的红棕榈象鼻虫中的生物学活性。通过剂量-死亡率生物测定、营养指数和本研究中特征基因的表达模式，研究了 novaluron 对 9 龄红棕榈象鼻虫幼虫的影响。实验室生物测定显示，9 龄红棕榈象鼻虫幼虫对 novaluron 的剂量依赖性死亡率反应，LD 为 14.77 ppm。用不同剂量的 novaluron（30、25、20、15、10 和 5 ppm）进行的膳食生长分析显示，与对照处理相比，其指数（如消化食物的转化率（82.38%）和摄入食物的转化率（74.27%））有非常高的降低。红棕榈象鼻虫幼虫的转录组分析表明，大量与几丁质降解有关的基因参与其中，包括 、 、 、 、 。然而，这些基因在 novaluron 喂养幼虫中的定量表达模式显示出组织特异性的时间依赖性表达模式。我们记录了从中肠组织过度表达所有基因。生长迟缓、几丁质重塑和杀幼虫潜能表明 novaluron 是一种有前途的替代管理方法。
