Rahmat Nur Lina, Zifruddin Anis Nadyra, Zainal Abidin Cik Mohd Rizuan, Nor Muhammad Nor-Azlan, Hassan Maizom
Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi 43600 UKM, Selangor, Malaysia.
Pest Management, FGV R&D Sdn. Bhd., Tun Razak 26400 PPP, Jengka, Pahang, Malaysia.
Genes (Basel). 2020 Dec 23;12(1):7. doi: 10.3390/genes12010007.
Bagworm, (Lepidoptera: Psychidae) is a ubiquitous insect pest in the oil palm plantations. infestation could reduce the oil palm productivity by 40% if it remains untreated over two consecutive years. Despite the urgency to tackle this issue, the genome and transcriptome of have not yet been fully elucidated. Here, we report a comprehensive transcriptome dataset from four different developmental stages of , comprising of egg, third instar larva, pupa and female adult. The de novo transcriptome assembly of the raw data had produced a total of 193,686 transcripts, which were then annotated against UniProt, NCBI non-redundant (NR) database, Gene Ontology, Cluster of Orthologous Group, and Kyoto Encyclopedia of Genes and Genomes databases. From this, 46,534 transcripts were annotated and mapped to 146 known metabolic or signalling KEGG pathways. The paper further identified 41 differentially expressed transcripts encoding seven genes in the chitin biosynthesis pathways, and their expressions across each developmental stage were further analysed. The genetic diversity of was profiled whereby there were 21,516 microsatellite sequences and 379,895 SNPs loci found in the transcriptome of . These datasets add valuable transcriptomic resources for further study of developmental gene expression, transcriptional regulations and functional gene activities involved in the development of . Identification of regulatory genes in the chitin biosynthesis pathway may also help in developing an RNAi-mediated pest control management by targeting certain pathways, and functional studies of the genes in .
蓑蛾（鳞翅目：蓑蛾科）是油棕种植园中普遍存在的害虫。如果连续两年不进行处理，虫害可能会使油棕产量降低40%。尽管解决这个问题刻不容缓，但蓑蛾的基因组和转录组尚未完全阐明。在此，我们报告了蓑蛾四个不同发育阶段（卵、三龄幼虫、蛹和雌成虫）的综合转录组数据集。对原始数据进行从头转录组组装共产生了193,686个转录本，然后将其与UniProt、NCBI非冗余（NR）数据库、基因本体论、直系同源簇和京都基因与基因组百科全书数据库进行比对注释。由此，46,534个转录本被注释并映射到146条已知的代谢或信号KEGG通路。该论文进一步鉴定了41个差异表达的转录本，它们编码几丁质生物合成途径中的7个基因，并进一步分析了它们在每个发育阶段的表达情况。对蓑蛾的遗传多样性进行了分析，在蓑蛾的转录组中发现了21,516个微卫星序列和379,895个单核苷酸多态性位点。这些数据集为进一步研究蓑蛾发育过程中的基因表达、转录调控和功能基因活性提供了宝贵的转录组资源。鉴定几丁质生物合成途径中的调控基因也可能有助于通过靶向某些途径开发RNAi介导的害虫防治管理方法，以及对蓑蛾中的基因进行功能研究。
