Blasco-Costa I, Seppälä K, Feijen F, Zajac N, Klappert K, Jokela J
Natural History Museum of Geneva, PO Box 6434, CH-1211Geneva 6, Switzerland.
Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Langnes, PO Box 6050, 9037Tromsø, Norway.
J Helminthol. 2019 Nov 29;94:e108. doi: 10.1017/S0022149X19000993.
The adult and metacercaria life stages of a new species of the microphallid genus Atriophallophorus Deblock & Rosé, 1964 are described from specimens collected at Lake Alexandrina (South Island, New Zealand). In addition to molecular analyses of ribosomal and mitochondrial genes, metacercariae of Atriophallophorus winterbourni n. sp. from the snail host Potamopyrgus antipodarum (Gray) were grown in vitro to characterize internal and external morphology of adults using light and scanning electron microscopy and histological techniques. Atriophallophorus winterbourni n. sp. is readily distinguishable from Atriophallophorus coxiellae Smith, 1973 by having a different structure of the prostatic chamber, sub-circular and dorsal to genital atrium, rather than cylindrical, fibrous, elongate and placed between the seminal vesicle and the genital atrium. The new species is most similar to Atriophallophorus minutus (Price, 1934) with regards to the prostatic chamber and the morphometric data, but possesses elongate-oval testes and subtriangular ovary rather than oval and transversely oval in A. minutus. Phylogenetic analyses including sequence data for A. winterbourni n. sp. suggested a congeneric relationship of the new species to a hitherto undescribed metacercariae reported from Australia, both forming a strongly supported clade closely related to Microphallus and Levinseniella. In addition, we provide an amended diagnosis of Atriophallophorus to accommodate the new species and confirm the sinistral interruption of the outer rim of the ventral sucker caused by the protrusion of the dextral parietal atrial scale at the base of the phallus.
1964年，德布洛克和罗塞描述了微茎科阿特里奥茎属的一个新物种，其成虫和尾蚴生活阶段是根据在亚历山德里纳湖（新西兰南岛）采集的标本描述的。除了对核糖体和线粒体基因进行分子分析外，还将来自蜗牛宿主新西兰扁卷螺（格雷）的温特伯恩阿特里奥茎新种尾蚴进行体外培养，以利用光学显微镜、扫描电子显微镜和组织学技术来表征成虫的内部和外部形态。温特伯恩阿特里奥茎新种很容易与1973年的考克斯阿特里奥茎区分开来，其前列腺腔结构不同，呈亚圆形，位于生殖窦背侧，而非圆柱形、纤维状、细长且位于精囊和生殖窦之间。就前列腺腔和形态测量数据而言，新物种与微小阿特里奥茎（普赖斯，1934年）最为相似，但具有长椭圆形睾丸和近三角形卵巢，而非微小阿特里奥茎中的椭圆形和横向椭圆形。包括温特伯恩阿特里奥茎新种序列数据的系统发育分析表明，该新物种与澳大利亚报道的一种迄今未描述的尾蚴存在同属关系，二者形成一个得到有力支持的分支，与微茎属和莱文森茎属密切相关。此外，我们对阿特里奥茎属进行了修订诊断，以纳入新物种，并确认了由于阴茎基部右侧顶叶心房鳞片突出而导致腹吸盘外缘左侧中断的情况。
