Martin Ivonne, Kaisar Maria M M, Wiria Aprilianto E, Hamid Firdaus, Djuardi Yenny, Sartono Erliyani, Rosa Bruce A, Mitreva Makedonka, Supali Taniawati, Houwing-Duistermaat Jeanine J, Yazdanbakhsh Maria, Wammes Linda J
Department of Mathematics, Faculty of Information Technology and Science, Parahyangan Catholic University, Bandung, Indonesia.
Department of Biomedical Data Sciences, section Medical Statistics, Leiden University Medical Center, Leiden, Netherlands.
Front Genet. 2019 Nov 6;10:1028. doi: 10.3389/fgene.2019.01028. eCollection 2019.
Soil-transmitted helminths have been shown to have the immune regulatory capacity, which they use to enhance their long term survival within their host. As these parasites reside in the gastrointestinal tract, they might modulate the immune system through altering the gut bacterial composition. Although the relationships between helminth infections or the microbiome with the immune system have been studied separately, their combined interactions are largely unknown. In this study we aim to analyze the relationship between bacterial communities with cytokine response in the presence or absence of helminth infections. For 66 subjects from a randomized placebo-controlled trial, stool and blood samples were available at both baseline and 21 months after starting three-monthly albendazole treatment. The stool samples were used to identify the helminth infection status and fecal microbiota composition, while whole blood samples were cultured to obtain cytokine responses to innate and adaptive stimuli. When subjects were free of helminth infection (helminth-negative), increasing proportions of was associated with lower levels of IL-10 response to LPS {estimate [95% confidence interval (CI)] -1.96 (-3.05, -0.87)}. This association was significantly diminished when subjects were helminth-infected (helminth positive) (-value for the difference between helminth-negative versus helminth-positive was 0.002). Higher diversity was associated with greater IFN-γ responses to PHA in helminth-negative (0.95 (0.15, 1.75); versus helminth-positive [-0.07 (-0.88, 0.73), -value = 0.056] subjects. Albendazole treatment showed no direct effect in the association between bacterial proportion and cytokine responses, although the ' effect on IL-10 responses to LPS tended downward in the albendazole-treated group [-1.74 (-4.08, 0.59)] versus placebo [-0.11 (-0.84, 0.62); -value = 0.193]. We observed differences in the relationship between gut microbiome composition and immune responses, when comparing individuals infected or uninfected with geohelminths. Although these findings are part of a preliminary exploration, the data support the hypothesis that intestinal helminths may modulate immune responses, in unison with the gut microbiota. ISRCTN, ISRCTN83830814. Registered 27 February 2008 - Retrospectively registered, http://www.isrctn.com/ISRCTN83830814.
土壤传播的蠕虫已被证明具有免疫调节能力,它们利用这种能力来提高在宿主体内的长期存活率。由于这些寄生虫寄生于胃肠道,它们可能通过改变肠道细菌组成来调节免疫系统。尽管蠕虫感染或微生物群与免疫系统之间的关系已分别进行了研究,但其联合相互作用在很大程度上尚不清楚。在本研究中,我们旨在分析在有或无蠕虫感染的情况下细菌群落与细胞因子反应之间的关系。对于一项随机安慰剂对照试验中的66名受试者,在基线以及开始每三个月服用阿苯达唑治疗21个月后均采集了粪便和血液样本。粪便样本用于确定蠕虫感染状况和粪便微生物群组成,而全血样本进行培养以获得对先天性和适应性刺激的细胞因子反应。当受试者无蠕虫感染(蠕虫阴性)时,[细菌种类]比例增加与对脂多糖(LPS)的白细胞介素-10(IL-10)反应水平降低相关{估计值[95%置信区间(CI)]-1.96(-3.05,-0.87)}。当受试者感染蠕虫(蠕虫阳性)时,这种关联显著减弱(蠕虫阴性与蠕虫阳性之间差异的P值为0.002)。在蠕虫阴性受试者中,较高的微生物多样性与对PHA的γ干扰素(IFN-γ)反应增强相关(0.95(0.15,1.75));而在蠕虫阳性受试者中则相反[-0.07(-0.88,0.73),P值 = 0.056]。阿苯达唑治疗在细菌比例与细胞因子反应之间的关联中未显示出直接作用,尽管在阿苯达唑治疗组中对LPS的IL-10反应的“[细菌种类]效应”呈下降趋势[-1.74(-4.08,0.59)],而安慰剂组为[-0.11(-0.84,0.62);P值 = 0.193]。在比较感染或未感染土源性蠕虫的个体时,我们观察到肠道微生物群组成与免疫反应之间的关系存在差异。尽管这些发现是初步探索的一部分,但数据支持这样的假设,即肠道蠕虫可能与肠道微生物群协同调节免疫反应。ISRCTN,ISRCTN83830814。于2008年2月27日注册 - 追溯注册,http://www.isrctn.com/ISRCTN83830814 。 (注:原文中“increasing proportions of ”处缺失具体内容,翻译时保留原文形式)