唾液中选定基质金属蛋白酶及其抑制剂水平与青少年特发性关节炎患者和健康对照者的口腔状况。
Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls.
机构信息
Department of Dentistry Propaedeutics, Medical University of Bialystok, ul. Szpitalna 30, 15-295 Bialystok, Poland.
Department of Integrated Dentistry, Medical University of Bialystok, Bialystok, Poland.
出版信息
Biomed Res Int. 2019 Oct 28;2019:7420345. doi: 10.1155/2019/7420345. eCollection 2019.
AIMS
Matrix metalloproteinases (MMPs) are a group of calcium-dependent zinc-containing proteinases acting both physiologically and in pathological conditions. The aim of this study was to evaluate the concentration of MMP-2, MMP-8, and MMP-9 and their inhibitors TIMP-1 and TIMP-2 of unstimulated whole saliva (UWS) in correlation with the oral health in juvenile idiopathic arthritis (JIA) children.
METHODS
The study population comprised 34 JIA patients and 34 age- and sex-matched controls (C). They were divided into two groups: with mixed dentition (MD) and with permanent dentition (PD). Dental caries (DMFT/dmft), unstimulated salivary flow rate (SF), and gingival inflammation (Gingival Index (GI) and Papilla Bleeding Index (PBI)) and oral hygiene (Simplified Oral Hygiene Index (OHI-S)) indices were evaluated. Saliva samples were tested with the enzyme-linked immunosorbent assay (ELISA) for MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2. Data were statistically analysed with the Mann-Whitney test and Spearman's rank correlation ( < 0.05).
RESULTS
There were no differences in dental hygiene or dental and periodontal status between the JIA and C groups. The MMP-9 concentration was higher in the whole JIA group compared with C (=0.005) and JIA MD groups (=0.038). A positive correlation of MMP-2 with the OHI-S index and a negative correlation of MMP-2 with SF were found in JIA. MMP-9 and its tissue inhibitor TIMP-1 had a positive mean correlation with the GI. A high correlation of MMP-8 with the number of decayed teeth (D) in JIA MD patients (=0.037) was revealed. In the JIA-PD patients, there was a positive correlation of MMP-2, -8, and -9 levels with gingival inflammation indices and a negative correlation of MMP-2 and 8 with the SF.
CONCLUSIONS
Despite a comparable clinical oral status of affected and unaffected children, in the JIA patients, a statistically significantly increased level of MMP-9 was found. In reference to the periodontal status, the role of MMPs increased in children with permanent dentition, whereas in reference to dental caries, the period of mixed dentition (MD) was critical.
目的
基质金属蛋白酶（MMPs）是一组依赖于钙和锌的蛋白水解酶，在生理和病理条件下均有作用。本研究旨在评估未刺激全唾液（UWS）中 MMP-2、MMP-8 和 MMP-9 及其抑制剂 TIMP-1 和 TIMP-2 的浓度与青少年特发性关节炎（JIA）儿童口腔健康的相关性。
方法
研究人群包括 34 名 JIA 患者和 34 名年龄和性别匹配的对照组（C）。他们被分为两组：混合牙列（MD）和恒牙列（PD）。评估了龋齿（DMFT/dmft）、未刺激唾液流速（SF）、牙龈炎症（Gingival Index（GI）和Papilla Bleeding Index（PBI））和口腔卫生（Simplified Oral Hygiene Index（OHI-S））指数。使用酶联免疫吸附试验（ELISA）检测唾液样本中的 MMP-2、MMP-8、MMP-9、TIMP-1 和 TIMP-2。使用曼-惠特尼 U 检验和斯皮尔曼等级相关分析（<0.05）对数据进行统计学分析。
结果
JIA 组和 C 组之间的口腔卫生或牙齿和牙周状况无差异。与 C（=0.005）和 JIA MD 组（=0.038）相比，整个 JIA 组 MMP-9 浓度更高。在 JIA 中，MMP-2 与 OHI-S 指数呈正相关，与 SF 呈负相关。MMP-9 和其组织抑制剂 TIMP-1 与 GI 呈正相关。在 JIA MD 患者中，MMP-8 与龋齿数（D）呈高度正相关（=0.037）。在 JIA-PD 患者中，MMP-2、-8 和 -9 水平与牙龈炎症指数呈正相关，与 SF 呈负相关。
结论
尽管受影响和未受影响儿童的临床口腔状况相当，但在 JIA 患者中，发现 MMP-9 水平显著升高。参考牙周状况，恒牙列中 MMPs 的作用增加，而参考龋齿，混合牙列（MD）时期是关键的。
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