Division of Oral Diagnostics and Rehabilitation, Department of Dental Medicine, Karolinska Institutet, and Scandinavian Center for Orofacial Neurosciences, 141 04, Huddinge, Sweden.
Department of Orofacial Pain and Jaw Function, Folktandvården Sörmland AB, Mälarsjukhuset, 611 32, Nyköping, Sweden.
Sci Rep. 2022 Feb 25;12(1):3240. doi: 10.1038/s41598-022-07233-0.
Monitoring the immune system's regulation and signaling using saliva could be of interest for clinicians and researchers. Saliva, a biofluid with close exchange with serum, is influenced by circadian variance and oral factors such as masticatory function. This study investigated the detectability and concentration of cytokines and chemokines in saliva in children with juvenile idiopathic arthritis (JIA) as well as saliva flow and the influence of orofacial pain on saliva flow. Of the 60 participants (7-14 years old) enrolled, 30 had a diagnosis of JIA and active disease, and 30 were sex- and age-matched healthy controls. Demographic data and three validated questions regarding presence of orofacial pain and dysfunction were recorded. Stimulated whole saliva was collected and analyzed using a customized R&D bead-based immunoassay with 21 targeted biomarkers. Fourteen of these were detectable and showed similar levels in both children with JIA and controls: TNF-alpha, TNFRSF1B, MMP-2, MMP-3, IL-1alpha, IL-1beta, IL-6R alpha, IL-8, S100A8, CCL2, CCL3, IL-10, CCL11, and CXCL9. In addition, there was no difference in salivary flow rate between groups, but there was an association between orofacial pain and reduced saliva flow rate for both groups.Trial registration: ClinicalTrials.gov Protocol id: 2010/2089-31/2.
使用唾液监测免疫系统的调节和信号对于临床医生和研究人员可能具有重要意义。唾液是一种与血清密切交换的生物流体，受昼夜节律变化和咀嚼功能等口腔因素的影响。本研究旨在探讨儿童青少年特发性关节炎（JIA）患者唾液中细胞因子和趋化因子的可检测性和浓度，以及唾液流率和口腔疼痛对唾液流率的影响。本研究共纳入 60 名参与者（7-14 岁），其中 30 名被诊断为 JIA 且疾病处于活动期，30 名年龄和性别匹配的健康对照组。记录了人口统计学数据和三个关于口腔疼痛和功能障碍的验证问题。收集并使用定制的 R&D 珠基免疫分析试剂盒分析刺激全唾液中的 21 种靶向生物标志物。其中 14 种可检测到，且在 JIA 患儿和对照组中水平相似：TNF-alpha、TNFRSF1B、MMP-2、MMP-3、IL-1alpha、IL-1beta、IL-6R alpha、IL-8、S100A8、CCL2、CCL3、IL-10、CCL11 和 CXCL9。此外，两组间唾液流率无差异，但两组均存在口腔疼痛与唾液流率降低相关。试验注册：ClinicalTrials.gov 方案 id：2010/2089-31/2。
