Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia.
Int J Mol Sci. 2019 Nov 27;20(23):5957. doi: 10.3390/ijms20235957.
A comparative study of the impact of n-butylamine and traditionally used additives (ammonium hydroxide and formic acid) on the efficiency of the electrospray ionization (ESI) process for the enhancement of metabolite coverage was performed by direct injection mass spectrometry (MS) analysis in negative mode. Evaluation of obtained MS data showed that n-butylamine is one of the most effective additives for the analysis of metabolite composition in ESI in negative ion mode (ESI(-)) The limitations of the use of n-butylamine and other alkylamines in the analysis of metabolic composition and a decontamination procedure that can reduce MS device contamination after their application are discussed. The proposed procedure allows the performance of high-sensitivity analysis of low-molecular-weight compounds on the same MS device in both polarities.
采用直接进样质谱（MS）分析，在负离子模式下进行了正丁胺与传统添加剂（氨水和甲酸）对电喷雾电离（ESI）过程增强代谢物覆盖效率影响的对比研究。对获得的 MS 数据的评估表明，正丁胺是用于 ESI 负离子模式（ESI(-)）中代谢物组成分析的最有效添加剂之一。讨论了在代谢物组成分析中使用正丁胺和其他烷基胺的局限性，以及一种去污程序，该程序可在使用后减少 MS 仪器的污染。所提出的程序允许在同一 MS 仪器上以两种极性对低分子量化合物进行高灵敏度分析。
