FFoQSI-Austrian Competence Centre for Feed and Food Quality, Safety & Innovation, Head Office, FFoQSI GmbH, Technopark 1C, 3430 Tulln, Austria.
Center of Excellence-Food Technology and Nutrition, University of Applied Sciences Upper Austria, 4600 Wels, Austria.
Molecules. 2019 Nov 27;24(23):4333. doi: 10.3390/molecules24234333.
Here, we report an accurate and versatile method for the simultaneous determination of 17 sugars (arabinose, erythrose, fructose, galactose, glucose, isomaltulose, lactose, lyxose, maltose, maltotriose, mannose, raffinose, rhamnose, ribose, sucrose, sorbose and xylose), seven polyols (erythritol, inositol, lactitol, maltitol, mannitol, sorbitol and xylitol), five ions (K, Br, Cl, NO and SO) and the pseudosaccharide acarbose. For compound separation, hydrophilic interaction chromatography (HILIC) coupled to a corona charged aerosol detector (CAD) was used. The method was validated for linearity, precision, reproducibility, retention factor and optimal injection volume. Standards were measured in the range of 1-1000 mg L and showed good intraday and interday repeatability, as well as precision (relative standard deviation (RSD) < 5%). The LODs and LOQs for the 30 analytes were in the range of 0.032-2.675 mg L and 0.107-8.918 mg L, respectively. This method exhibited correlation coefficients of at least R > 0.97 for all analytes. The method was tested in 24 food and beverage samples to validate the separation efficiency and sensitivity in natural food matrices and to show the practicability of its use for routine food analysis.
在这里，我们报告了一种准确且通用的方法，可同时测定 17 种糖（阿拉伯糖、赤藓糖、果糖、半乳糖、葡萄糖、异麦芽酮糖、乳糖、木酮糖、麦芽糖、麦芽三糖、甘露糖、棉子糖、鼠李糖、核糖、蔗糖、山梨醇和木糖）、7 种多元醇（赤藓糖醇、肌醇、乳糖醇、麦芽糖醇、甘露醇、山梨糖醇和木糖醇）、5 种离子（K、Br、Cl、NO 和 SO）和假糖阿卡波糖。为了进行化合物分离，采用亲水相互作用色谱（HILIC）与电喷雾检测器（CAD）联用。该方法对线性、精密度、重现性、保留因子和最佳进样量进行了验证。标准品在 1-1000 mg L 的范围内进行了测量，显示出良好的日内和日间重复性以及精密度（相对标准偏差（RSD）<5%）。30 种分析物的检出限和定量限分别在 0.032-2.675 mg L 和 0.107-8.918 mg L 的范围内。该方法对所有分析物的相关系数至少为 R > 0.97。该方法在 24 种食品和饮料样品中进行了测试，以验证其在天然食品基质中的分离效率和灵敏度，并展示其在常规食品分析中的实用性。
