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糯米与茯砖茶的多菌种发酵:对微生物、化学和挥发性成分的影响。

Multistarter fermentation of glutinous rice with Fu brick tea: Effects on microbial, chemical, and volatile compositions.

机构信息

College of Life Science, Shaoxing University, Shaoxing, Zhejiang 312000, China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.

College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

出版信息

Food Chem. 2020 Mar 30;309:125790. doi: 10.1016/j.foodchem.2019.125790. Epub 2019 Nov 26.

Abstract

A higher fermentation efficiency was achieved, using multistarter fermentation of glutinous rice supplemented with Fu brick tea (FGR-FBT), than when using traditional fermentation. The effects of multistarter fermentation on the microbial, chemical, and volatile compositions were determined. When FBT was incorporated during glutinous rice fermentation, increased population of yeasts and fungi, as well as enhanced α-amylase, proteinase and β-glucosidase activities, were observed. Specific fungi were isolated and identified as Aspergillus spp., which are known to secrete extracellular enzymes that modify the chemical properties, including ethanol levels, pH, total acids, and total soluble solids. The aroma profile of fermented glutinous rice was studied in the absence and presence of FBT, using HS-SPME-GC-MS and the electronic-nose. This analysis indicated that 35 characteristic volatile compounds were only found in FGR-FBT. The results show that FBT can be added during the fermentation of food products to enhance microbial biotransformation and modify flavour metabolism.

摘要

采用添加茯砖茶的多菌种发酵工艺(FGR-FBT)对糯米进行发酵,比传统发酵方法具有更高的发酵效率。本文研究了多菌种发酵对微生物、化学和挥发性成分的影响。结果表明,在糯米发酵过程中添加茯砖茶会增加酵母和真菌的种群数量,并提高α-淀粉酶、蛋白酶和β-葡萄糖苷酶的活性。分离并鉴定出特定的真菌为曲霉菌属,其分泌的胞外酶可以改变化学性质,包括乙醇水平、pH 值、总酸和总可溶固形物。使用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)和电子鼻分析了在有无茯砖茶存在的情况下发酵糯米的香气特征。结果表明,只有在 FGR-FBT 中才发现 35 种特征挥发性化合物。这些结果表明,在发酵食品的过程中可以添加茯砖茶,以增强微生物的生物转化作用并改变风味代谢。

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