Department of Cardiothoracic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
Department of Cardiothoracic Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
Cytokine. 2020 Mar;127:154920. doi: 10.1016/j.cyto.2019.154920. Epub 2019 Nov 28.
Stanford type A Aortic dissection (TAAD) is a deadly cardiovascular disease but the relationship between inflammatory cytokines and disease pathogenesis is still unclear. Observation of the changes of different chemokines may help to explore the etiology of TAAD much further. Clinical data was collected from TAAD patients (TAAD group) and healthy controls (HC group) in our institute between October 2013 and December 2014. Blood sample was harvested from each subject of two groups. The expression levels of eighty chemokines were examined by protein array technology. Then we tested the expressions of macrophage inflammatory protein 1β (MIP-1β), epithelial neutrophil activating peptide 78 (ENA-78), interleukin 16 (IL-16), interferon inducible protein 10 (IP-10), and FMS-like tyrosine kinase 3 (Flt-3) ligand by using luminex technology. Osteopontin (OPN) and monocyte chemotaxis protein (MCP) levels were analyzed by ELISA kits. The mean age of TAAD group is 49.9 ± 11.2 and 48.7 ± 9.9 in HC group, respectively. 76.0% of TAAD patients and 72.0% of healthy controls were male. MIP-1β and ENA-78 expression in TAAD group were significantly lower than that in HC group, while significant increasing IL-16 level was found. Plasma levels of OPN in TAAD group increased remarkably compared with HC group, but MCP-1 and MCP-2 expression significantly decreased. No correlation was shown between serum CRP levels and plasma level of these cytokines by using Spearman analysis. ROC analysis showed that OPN could be indicators for TAAD diagnosis with sensitivity of 0.92 and specificity of 0.99. Our results provide a reasonable way to focus on the chemokines in understanding the pathogenesis of human TAAD.
斯坦福 A 型主动脉夹层（TAAD）是一种致命的心血管疾病，但炎症细胞因子与疾病发病机制之间的关系仍不清楚。观察不同趋化因子的变化可能有助于进一步探讨 TAAD 的病因。本研究收集了 2013 年 10 月至 2014 年 12 月我院 TAAD 患者（TAAD 组）和健康对照者（HC 组）的临床资料。采集两组受试者血样，应用蛋白芯片技术检测 80 种趋化因子的表达水平，然后采用 Luminex 技术检测巨噬细胞炎性蛋白 1β（MIP-1β）、上皮中性粒细胞激活肽 78（ENA-78）、白细胞介素 16（IL-16）、干扰素诱导蛋白 10（IP-10）、FMS 样酪氨酸激酶 3（Flt-3）配体的表达水平，酶联免疫吸附试验（ELISA）试剂盒检测骨桥蛋白（OPN）和单核细胞趋化蛋白（MCP）的水平。TAAD 组平均年龄为 49.9±11.2 岁，HC 组为 48.7±9.9 岁；TAAD 组和 HC 组患者中，76.0%和 72.0%为男性。TAAD 组 MIP-1β 和 ENA-78 的表达明显低于 HC 组，而 IL-16 水平显著升高。TAAD 组 OPN 水平明显高于 HC 组，而 MCP-1 和 MCP-2 表达明显降低。Spearman 分析显示，血清 CRP 水平与这些细胞因子的血浆水平之间无相关性。ROC 分析显示，OPN 可作为 TAAD 诊断的指标，其敏感度为 0.92，特异度为 0.99。我们的研究结果为关注趋化因子在理解人类 TAAD 发病机制方面提供了合理的思路。
