Fan F D, Xu Z J, Zhou Q, Wang D J
Department of Cardiothoracic Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2017 Apr 24;45(4):318-322. doi: 10.3760/cma.j.issn.0253-3758.2017.04.012.
To explore the plasma chemokines expressions and related clinical implication in patients with Stanford type A aortic dissection (AD). We retrospectively analyzed the data of 65 patients with Stanford type A aortic dissection, hypertensive patients and 11 healthy subjects admitted in our department from October 2013 to December 2014, they were divided into four groups: NH-CON group (11 healthy subjects), H-AD group (29 AD patients with hypertension), NH-AD group (21 AD patients without hypertension), and H-CON group (14 hypertension patients). Four plasma samples from AD patients and 4 plasma samples from healthy subjects were collected randomly with random numbers table, and the levels of different chemokines were examined by protein array analysis. Then, plasma levels of chemokines including macrophage inflammatory protein 1β(MIP-1β), epithelial neutrophil activating peptide 78(ENA-78), interleukin 16(IL-16), interferon inducible protein 10(IP-10) and FMS-like tyrosine kinase 3(Flt-3) ligand were analyzed by luminex. Pearson analysis was used to determine the correlations between the chemokines and serum C reactive protein (CRP) levels. Plasma levels of MIP-1β(34.0(29.3, 47.2) ng/L vs. 51.0(28.2, 80.7) ng/L, <0.05) and ENA-78(110.5(59.1, 161.4) ng/L vs. 475.7(299.3, 837.3) ng/L, <0.05) were significantly lower in H-AD group, while plasma IL-16 level was significantly higher in H-AD group(54.7(16.3, 187.8) ng/L vs. 17.5(11.9, 20.8) ng/L, <0.05) than in H-CON group. Plasma levels of MIP-1β(48.3(26.4, 62.1) ng/L, <0.05) were significantly lower in H-AD patients than in NH-AD patients. Plasma level of ENA-78 was significantly lower in NH-AD group than in NH-CON group (95.0(58.0, 155.0) ng/L vs. 257.7(85.2, 397.8) ng/L, <0.05). The levels of IP-10 and Flt-3 ligand were similar among the 4 groups (all >0.05). Pearson analysis showed that there were no correlation between MIP-1β((2)=0.01, >0.05), ENA-78((2)=0.02, >0.05), IL-16((2)=0.02, >0.05), IP-10((2)=0.00, >0.05), Flt-3 ligand((2)=0.02, >0.05) and CRP levels in patients with Stanford type A aortic dissection. Lower plasma levels of MIP-1β and ENA-78 and higher plasma levels of IL-16 may associate with the occurrence and development of type A aortic dissection, but their concentrations are not correlated with serum CRP levels. There is no significant change on plasma levels of IP-10 and Flt-3 in the Stanford type A aortic dissection patients.
探讨A型主动脉夹层（AD）患者血浆趋化因子的表达及相关临床意义。我们回顾性分析了2013年10月至2014年12月在我科收治的65例A型主动脉夹层患者、高血压患者及11例健康受试者的数据，将其分为四组：NH-CON组（11例健康受试者）、H-AD组（29例合并高血压的AD患者）、NH-AD组（21例未合并高血压的AD患者）和H-CON组（14例高血压患者）。采用随机数字表法从AD患者中随机抽取4份血浆样本，从健康受试者中随机抽取4份血浆样本，通过蛋白质芯片分析检测不同趋化因子水平。然后，采用Luminex法分析血浆中巨噬细胞炎性蛋白1β（MIP-1β）、上皮中性粒细胞激活肽78（ENA-78）、白细胞介素16（IL-16）、干扰素诱导蛋白10（IP-10）和FMS样酪氨酸激酶3（Flt-3）配体等趋化因子水平。采用Pearson分析确定趋化因子与血清C反应蛋白（CRP）水平之间的相关性。H-AD组血浆MIP-1β水平（34.0（29.3，47.2）ng/L vs. 51.0（28.2，80.7）ng/L，<0.05）和ENA-78水平（110.5（59.1，161.4）ng/L vs. 475.7（299.3，837.3）ng/L，<0.05）显著低于H-CON组，而H-AD组血浆IL-16水平显著高于H-CON组（54.7（16.3，187.8）ng/L vs. 17.5（11.9，20.8）ng/L，<0.05）。H-AD患者血浆MIP-1β水平（48.3（26.4，62.1）ng/L，<0.05）显著低于NH-AD患者。NH-AD组血浆ENA-78水平显著低于NH-CON组（95.0（58.0，155.0）ng/L vs. 257.7（85.2，397.8）ng/L，<0.05）。四组间IP-10和Flt-3配体水平相似（均P>0.05）。Pearson分析显示，A型主动脉夹层患者中MIP-1β（r=0.01，P>0.05）、ENA-78（r=0.02，P>o.o5）、IL-16（r=0.02，P=o.o5）、IP-10（r=0.00，P>0.05）、Flt-3配体（r=0.02，P>0.05）与CRP水平均无相关性。血浆MIP-1β和ENA-78水平降低及IL-16水平升高可能与A型主动脉夹层的发生发展有关，但其浓度与血清CRP水平无关。A型主动脉夹层患者血浆IP-10和Flt-3水平无明显变化。
