Cottingham I R, Moore A L
Department of Biochemistry, University of Sussex, Brighton, U.K.
Biochem J. 1988 Aug 15;254(1):303-5. doi: 10.1042/bj2540303.
The present paper describes the analysis of plant mitochondrial NADH dehydrogenases using a recently developed non-dissociating gradient polyacrylamide-gel-electrophoresis technique [Kuonen, Roberts & Cottingham (1986) Anal. Biochem. 153, 221-226]. Solubilized mung-bean (Phaseolus aureus) submitochondrial particles were analysed on 3-22% (w/v) gradient polyacrylamide gels containing 0.1% Triton X-100 and stained for multiple NADH dehydrogenase activities. A rotenone-sensitive NADH dehydrogenase (Complex I) was identified on the basis of co-migration with the purified mammalian enzyme. The polypeptide composition of the plant enzyme was further analysed by band excision and SDS/polyacrylamide-gel electrophoresis.
本文描述了使用最近开发的非解离梯度聚丙烯酰胺凝胶电泳技术[库嫩、罗伯茨和科廷厄姆（1986年）《分析生物化学》153卷，221 - 226页]对植物线粒体NADH脱氢酶进行的分析。在含有0.1% Triton X - 100的3 - 22%（w/v）梯度聚丙烯酰胺凝胶上分析溶解的绿豆（菜豆）亚线粒体颗粒，并对多种NADH脱氢酶活性进行染色。根据与纯化的哺乳动物酶的共迁移情况鉴定出一种对鱼藤酮敏感的NADH脱氢酶（复合体I）。通过条带切除和SDS/聚丙烯酰胺凝胶电泳进一步分析了植物酶的多肽组成。
