用[3H]二氢鱼藤酮对NADH脱氢酶的线粒体编码亚基进行光标记。
Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone.
作者信息
Earley F G, Patel S D, Ragan I, Attardi G
出版信息
FEBS Lett. 1987 Jul 13;219(1):108-12. doi: 10.1016/0014-5793(87)81200-0.
PMID:3297786
Abstract
Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of Mr 33,000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND-1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C-terminal sequence of the human ND-1 gene product.
摘要
牛心线粒体NADH脱氢酶用抑制剂[3H]二氢鱼藤酮进行光标记。该酶疏水结构域中分子量为33,000的一种成分是主要的标记位点。通过用针对人ND-1基因产物预期C末端序列产生的抗血清进行免疫印迹和免疫沉淀，确定了该蛋白质与线粒体编码的ND-1基因产物的一致性。
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