L1210白血病细胞对肌醇的摄取与代谢
Uptake and metabolism of myo-inositol by L1210 leukaemia cells.
作者信息
Moyer J D, Malinowski N, Napier E A, Strong J
机构信息
Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, MD 20892.
出版信息
Biochem J. 1988 Aug 15;254(1):95-100. doi: 10.1042/bj2540095.
The initial rate of uptake of [3H]myo-inositol by L1210 murine leukaemia cells is directly proportional to the extracellular concentration and unaffected by several analogues of myo-inositol even at millimolar concentrations. Scyllitol, a geometric isomer of myo-inositol, partially inhibited the uptake of myo-inositol (40% at 0.1 mM). A portion of the uptake of myo-inositol was not inhibited even at 5 mM-scyllitol. At steady-state the intracellular concentration of [3H]myo-inositol is directly proportional to the extracellular concentration. Addition of myo-inositol to medium does not enhance the growth of L1210 cells; these cells can maintain an extracellular concentration of 20 microM-myo-inositol even when grown in myo-inositol-free medium. Synthesis of myo-inositol from glucose by L1210 cells was demonstrated by use of [13C]glucose and m.s. L1210 cells maintain myo-inositol pools by a combination of synthesis de novo and uptake of exogenous myo-inositol by either passive diffusion or a low affinity carrier.
L1210小鼠白血病细胞对[3H]肌醇的初始摄取速率与细胞外浓度成正比，即使在毫摩尔浓度下，也不受几种肌醇类似物的影响。肌醇的几何异构体鲨肌醇部分抑制了肌醇的摄取（0.1 mM时为40%）。即使在5 mM鲨肌醇的情况下，一部分肌醇摄取也未被抑制。在稳态时，[3H]肌醇的细胞内浓度与细胞外浓度成正比。向培养基中添加肌醇不会促进L1210细胞的生长；即使在无肌醇的培养基中生长，这些细胞也能维持20 μM肌醇的细胞外浓度。通过使用[13C]葡萄糖和质谱法证明了L1210细胞从葡萄糖合成肌醇。L1210细胞通过从头合成和通过被动扩散或低亲和力载体摄取外源性肌醇的组合来维持肌醇池。
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