吲哚美辛对体外T细胞和B细胞活化及细胞介导的细胞溶解的影响。

Effect of indomethacin on in vitro T- and B-cell activation and cell-mediated lysis.

作者信息

Barasoain I, Rojo J M, Portolés A

出版信息

Immunopharmacology. 1979 Dec;2(1):83-92. doi: 10.1016/0162-3109(79)90023-7.

Abstract

The effect of indomethacin, an inhibitor of prostaglandin synthetase, on lymphocyte blast transformation induced by T- or B-cell activators has been studied. Simultaneously adding indomethacin (0.03-0.3 x 10(-6) M final concentration) and concanavalin A to mouse spleen cell cultures, led to an enhancement of 3H-thymidine uptake, whereas 30 x 10(-6) M indomethacin inhibited this uptake. The stimulation induced by indomethacin was higher when this drug was present in the cultures before the addition of the mitogen. Neither the optimal concanavalin A concentration nor the day on which the maximum of 3H-thymidine uptake occurred were altered by indomethacin. Activation of B lymphocytes induced by bacterial lipopolysaccharide was inhibited by indomethacin at all concentrations tested. However, indomethacin similarly blocked the prostaglandin synthesis as well in lipopolysaccharide- or concanavalin A-stimulated lymphocyte cultures. Indomethacin enhanced the one-way mixed lymphocyte reaction. No significant effect of indomethacin was found on cell-mediated cytotoxicity of 51Cr labeled targets. The results are discussed in terms of differential sensitivity of B and T lymphocytes to this anti-inflammatory drug.

摘要

研究了前列腺素合成酶抑制剂吲哚美辛对T细胞或B细胞激活剂诱导的淋巴细胞增殖转化的影响。在小鼠脾细胞培养物中同时加入吲哚美辛（终浓度为0.03 - 0.3×10⁻⁶ M）和刀豆球蛋白A，可使³H-胸腺嘧啶核苷摄取增加，而30×10⁻⁶ M的吲哚美辛则抑制这种摄取。当在加入丝裂原之前将该药物加入培养物中时，吲哚美辛诱导的刺激作用更强。吲哚美辛既未改变刀豆球蛋白A的最佳浓度，也未改变³H-胸腺嘧啶核苷摄取量达到最大值的天数。在所有测试浓度下，吲哚美辛均抑制细菌脂多糖诱导的B淋巴细胞激活。然而，吲哚美辛在脂多糖或刀豆球蛋白A刺激的淋巴细胞培养物中同样能阻断前列腺素的合成。吲哚美辛增强了单向混合淋巴细胞反应。未发现吲哚美辛对⁵¹Cr标记靶细胞的细胞介导细胞毒性有显著影响。根据B淋巴细胞和T淋巴细胞对这种抗炎药物的不同敏感性对结果进行了讨论。