Karamany R M, Kazar J, Malik S A, al-Mufti S, Badriya M
Department of Virology, Public Health Laboratories, Kuwait.
APMIS Suppl. 1988;3:40-3.
An improved method of ELISA for rabies post-vaccination antibody determination has been developed comparing adsorption properties of polystyrene beads and microtitre plates which were coated with different concentrations of rabies virus antigen. All 106 human post-vaccination serum samples tested were found repeatedly positive within the range of mean values (MV) from 1.4 to 43.0 international units (IU)/0.1 ml. The plates displayed much higher coefficient of variation (CV) when testing lower serum dilutions in different wells of the same plate. In higher dilutions, the sera with high and low antibody levels could easily be distinguished, the results corresponding well to those of the indirect fluorescent antibody test (IFAT), with the exception of some negative sera also to those obtained by ELISA at the Institut Pasteur (Paris). The slightest CV occurred with a 1:400 serum dilution on beads, indicating that ELISA using beads as solid phase can be recommended as a rapid, sensitive and reliable technique for quantitative assay of rabies post-vaccination antibody.
已开发出一种改进的酶联免疫吸附测定（ELISA）方法，用于测定狂犬病疫苗接种后的抗体，该方法比较了涂有不同浓度狂犬病病毒抗原的聚苯乙烯珠和微量滴定板的吸附特性。在测试的所有106份人类疫苗接种后血清样本中，发现其平均值（MV）在1.4至43.0国际单位（IU）/0.1毫升范围内均呈反复阳性。在同一板的不同孔中测试较低血清稀释度时，平板显示出更高的变异系数（CV）。在较高稀释度下，高抗体水平和低抗体水平的血清很容易区分，结果与间接荧光抗体试验（IFAT）的结果非常吻合，但一些阴性血清除外，巴黎巴斯德研究所通过ELISA获得的结果也除外。在珠子上以1:400血清稀释度时变异系数最小，这表明使用珠子作为固相的ELISA可作为一种快速、灵敏且可靠的技术，用于狂犬病疫苗接种后抗体的定量测定。
