St. Joseph's College of Post graduate and Research Studies, Bangalore, 560027, India.
St. Joseph's College of Post graduate and Research Studies, Bangalore, 560027, India.
Microb Pathog. 2020 Feb;139:103805. doi: 10.1016/j.micpath.2019.103805. Epub 2019 Nov 29.
Studies on understanding the human microbiome continue to grow rapidly; nonetheless, reports on alterations in the microbiome post HIV infection are limited. Human microbiome is an aggregate of bacteria, fungi, viruses and archaea that have co-evolved with humans. These microbes have important roles in immune modulation, vitamin synthesis, metabolism etc. The human pharyngeal microbiome, which resides in the junction between digestive and respiratory tracts, might have a key role in the prevention of respiratory tract infections, akin to the actions of the intestinal microbiome against enteric infections. The respiratory tract is constantly exposed to various environmental and endogenous microbes; however, unlike other similar mucosal surfaces, there has been limited investigation of the microbiome of the respiratory tract. HIV infection is associated with alterations in the respiratory microbiome. The aim of this study was to use next-generation sequencing to determine the composition of the oropharyngeal microbiome in a HIV-positive individual. The bacterial composition was determined by illumina sequencing using MiSeq of partial 16S rRNA genes (V3-V4). A total of 3, 57,926 reads were analyzed. Overall, the genera Proteus, Enterococcus, Bacteroides, Prevotella and Clostridium were most prevalent bacterial populations in the oropharynx of an HIV positive patient.
关于人类微生物组的研究仍在迅速发展；尽管如此，关于 HIV 感染后微生物组变化的报告却很有限。人类微生物组是与人类共同进化的细菌、真菌、病毒和古菌的集合体。这些微生物在免疫调节、维生素合成、代谢等方面发挥着重要作用。位于消化道和呼吸道交界处的咽微生物组，可能在预防呼吸道感染方面发挥着关键作用，就像肠道微生物组对抗肠道感染一样。呼吸道不断暴露于各种环境和内源性微生物中；然而，与其他类似的黏膜表面不同，对呼吸道微生物组的研究有限。HIV 感染与呼吸道微生物组的变化有关。本研究旨在使用下一代测序技术来确定 HIV 阳性个体的口咽微生物组的组成。细菌组成通过 MiSeq 用 illumina 测序用部分 16S rRNA 基因（V3-V4）确定。共分析了 357926 个读数。总体而言，变形菌属、肠球菌属、拟杆菌属、普雷沃氏菌属和梭菌属是 HIV 阳性患者口咽部位最常见的细菌种群。
