College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Microb Pathog. 2020 Feb;139:103899. doi: 10.1016/j.micpath.2019.103899. Epub 2019 Nov 29.
A Staphylococcus aureus (S.aureus) was isolated from pigs suffered in pneumonia that can't be cured by antibiotic such as methicillin and vancomycin. It was demonstrated that baicalin, an active natural compound extracted from the traditional Chinese medicinal, possess antimicrobial activity. In the present study, we evaluate it efficacy in vitro and vivo against this isolated methicillin-resistant S.aureus (MRSA). Our findings demonstrated that baicalin can inhibit S. aureus growth in a dose-dependent manner and attenuate the biofilm formation. Scanning electron microscopies showed that cell membrane was damaged and accompany with contents leaks after treated with high concentration of baicalin. In addition, baicalin exerted inhibitory effects on the expression of S.aureus virulence-related factors. Moreover, baicalin treated mice had enhanced survival after a lethal dose of S.aureus infection compared with untreated mice. Simultaneously, the pathological tissue damage and bacterium burden were decrease in baicalin treated mice. These data demonstrated that baicalin displayed a high effectiveness in vitro and vivo against MRSA infection, suggesting that baicalin may potentially be used to treat MRSA infection.
从无法用抗生素（如甲氧西林和万古霉素）治愈肺炎的猪中分离出一株金黄色葡萄球菌（S.aureus）。研究表明，黄芩苷是一种从传统中药中提取的活性天然化合物，具有抗菌活性。本研究评估了其对这种分离的耐甲氧西林金黄色葡萄球菌（MRSA）的体外和体内疗效。研究结果表明，黄芩苷可以抑制金黄色葡萄球菌的生长，呈剂量依赖性，并减弱生物膜的形成。扫描电子显微镜显示，用高浓度黄芩苷处理后，细胞膜被破坏，并伴有内容物泄漏。此外，黄芩苷对金黄色葡萄球菌毒力相关因子的表达也有抑制作用。此外，黄芩苷处理的小鼠在致死剂量金黄色葡萄球菌感染后存活率提高，与未处理的小鼠相比。同时，黄芩苷处理的小鼠的组织病理损伤和细菌负荷减少。这些数据表明，黄芩苷对 MRSA 感染具有很高的体外和体内疗效，表明黄芩苷可能具有治疗 MRSA 感染的潜力。
