Grzelka Kajetan, Matkowski Adam, Ślusarczyk Sylwester
Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland.
Botanical Garden of Medicinal Plants of the Wroclaw Medical University, Wroclaw, Poland.
Front Plant Sci. 2023 Mar 1;14:1142624. doi: 10.3389/fpls.2023.1142624. eCollection 2023.
Georgi is a valuable medicinal plant of the family. The roots, , are valued in the traditional medicine of East Asia and are also listed in several pharmacopeias, such as the Chinese and European versions. The roots contain a high amount of flavones, such as baicalein, wogonin and their glucuronides, baicalin and wogonoside, respectively, with rare structures of unsubstituted B-ring. These major constituents are responsible for its pharmacological activity, mainly anti-inflammatory, antiviral, and antitumor, as well as BDZ-receptor modulating. There is a fast-growing demand for both the crude drug and the individual flavonoids obtained from it. However, the variability of content and composition of flavonoids in the roots is significant and affects pharmaceutical use, and little is known about the influence of various factors on root quality. In our experiments, we use aeroponics to determine the effect of electroporation as an abiotic stressor on plant growth, development, and root mass, as well as on its metabolic profile.
Electroporation significantly impacted plant growth and the content of flavonoids, especially baicalein and wogonin, depending on the treatment parameters. Concentrations of aglycones were increased in at least half of the treatment conditions. The greatest amounts (a 2.5-fold increase compared to controls) were recorded after applying an electrical field characterized by the following parameters: E = 3 kV/cm, t = 100 μs, and N = 10. In conclusion, electrostimulation is an innovative and efficient way to increase plant growth and yield in an aeroponic system, as well as modulate the profile and content of bioactive flavones in the roots. However, the fine-tuning of these parameters, such as the electrical field strength (E), length (t), and number (N) of impulses delivered, is of great importance. It was also shown that cultivation of the experimental plants in aeroponics had a positive impact on their survival and development while being a sustainable and efficient horticultural practice.
Georgi是该科一种有价值的药用植物。其根在东亚传统医学中备受重视，并且也被列入了几部药典，如中国版和欧洲版。根中含有大量黄酮类化合物，如黄芩素、汉黄芩素及其葡萄糖醛酸苷，分别为黄芩苷和汉黄芩苷，具有罕见的未取代B环结构。这些主要成分决定了其药理活性，主要是抗炎、抗病毒和抗肿瘤，以及调节BDZ受体。对其粗药和从中获得的单个黄酮类化合物的需求都在快速增长。然而，根中黄酮类化合物的含量和组成差异很大，影响了药物使用，而且对于各种因素对根质量的影响知之甚少。在我们的实验中，我们使用气培法来确定电穿孔作为一种非生物胁迫因素对植物生长、发育和根质量以及其代谢谱的影响。
根据处理参数，电穿孔对植物生长和黄酮类化合物含量，尤其是黄芩素和汉黄芩素，有显著影响。在至少一半的处理条件下，苷元浓度有所增加。在施加具有以下参数的电场后记录到了最大量（与对照相比增加了2.5倍）：E = 3 kV/cm，t = 100 μs，N = 10。总之，电刺激是一种在气培系统中增加植物生长和产量以及调节根中生物活性黄酮类化合物谱和含量的创新且高效的方法。然而，对这些参数进行微调，如电场强度（E）、脉冲长度（t）和施加的脉冲数（N），非常重要。还表明，在气培中种植实验植物对其存活和发育有积极影响，同时也是一种可持续且高效的园艺实践。
