Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil; Paulo Picanço School of Dentistry, Fortaleza, Brazil.
Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, Brazil; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
Dent Mater. 2020 Jan;36(1):e1-e8. doi: 10.1016/j.dental.2019.11.019. Epub 2019 Nov 30.
To investigate the effects of natural collagen cross-linkers incorporation in phosphoric acid etchant on dentin biomodification, microtensile bond strength (μTBS) and nanoleakage (NL) of a two-step etch-and-rinse adhesive.
Experimental aqueous solution of 37% ortho-phosphoric acid were prepared with the addition of 2% biomodification agents: Lignin (LIG) from industrial paper production residue, Cardanol (CARD) from cashew-nut shell liquid, and Proanthocyanidin (PAC) from grape-seed extract. Negative control (NC) was acid solution without cross-linker whilst commercial control (CC) was Condac 37 gel (FGM). Dentin specimens were assayed by FTIR after 15s etching to detect collagen cross-linking. Extracted third molars were used for μTBS (n=7) and fracture mode analysis of Optibond S (Kerr), tested after 24h or 1000 thermal cycles. NL was surveyed by SEM. Statistical analysis was performed with two-way ANOVA and Tukey's test (p<0.05).
FTIR confirmed cross-linking for all agents. μTBS of CC was the highest (46.6±6.2MPa), but reduced significantly after aging (35.7±5.2MPa) (p<0.001). LIG (30.6±3.7MPa) and CARD (28.3±1.8MPa) attained similar μTBS which were stable after aging (p>0.05). Fracture mode was predominantly adhesive. At 24h, all groups showed presence of silver uptake in hybrid layer, except CARD. After aging, CARD- and LIG-treated specimens exhibited little amount of silver penetration. CC, PAC and NC showed gaps, great nanoleakage at hybrid layer and presence of water channels in adhesive layer.
Altogether, ortho-phosphoric acid incorporated with LIG and CARD promotes stable resin-dentin bond strength with minor nanoleakage after aging, thereby achieving therapeutic impact without additional clinical steps.
研究在磷酸酸蚀剂中加入天然胶原交联剂对牙本质生物改性、微拉伸结合强度（μTBS）和两步酸蚀-冲洗型胶粘剂纳米渗漏（NL）的影响。
制备 37%邻苯磷酸酸蚀实验液，加入 2%生物改性剂：来自工业造纸废料的木质素（LIG）、来自腰果壳液的腰果酚（CARD）和来自葡萄籽提取物的原花青素（PAC）。阴性对照（NC）为不含交联剂的酸溶液，而商业对照（CC）为 Condac 37 凝胶（FGM）。用傅里叶变换红外光谱（FTIR）检测 15 秒蚀刻后牙本质的胶原交联情况。提取第三磨牙进行 Optibond S（Kerr）的 μTBS（n=7）和断裂模式分析，24 小时或 1000 次热循环后进行测试。NL 通过扫描电子显微镜（SEM）进行调查。采用双因素方差分析和 Tukey 检验（p<0.05）进行统计分析。
FTIR 证实所有试剂均发生交联。CC 的 μTBS 最高（46.6±6.2MPa），但老化后显著降低（35.7±5.2MPa）（p<0.001）。LIG（30.6±3.7MPa）和 CARD（28.3±1.8MPa）的 μTBS 相似，且老化后稳定（p>0.05）。断裂模式主要为黏附性。24 小时时，除 CARD 外，所有组的混合层均有银摄取。老化后，CARD 和 LIG 处理的标本显示银渗透量较少。CC、PAC 和 NC 显示混合层有间隙、NL 较大，黏附层有水流通道。
总之，邻苯磷酸酸蚀剂中加入 LIG 和 CARD 可在不增加临床步骤的情况下促进稳定的树脂-牙本质粘结强度，并具有较小的 NL，从而达到治疗效果。
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