1 型糖尿病小鼠模型中的 MAIT 细胞。

MAIT Cells in Type 1 Diabetes Mouse Models.

机构信息

INSERM U1016, Institut Cochin, Paris, France.

CNRS, UMR8104, Paris, France.

出版信息

Methods Mol Biol. 2020;2098:283-297. doi: 10.1007/978-1-0716-0207-2_18.

DOI:10.1007/978-1-0716-0207-2_18

PMID:31792829

Abstract

MAIT cells are unconventional T cells expressing a semi-invariant αβ TCR, and they recognize bacterial metabolites via the highly conserved MR1 protein. MAIT cells interact with gut microbiota and literature reports alterations of gut homeostasis in type 1 diabetes (T1D), suggesting the involvement of MAIT cells in T1D. Since NOD mice is a well-established mouse model of T1D, MAIT cells were studied in these mice to evaluate their potential involvement in disease development. This chapter describes the material and methods required to characterize MAIT cells and to determine their function in T1D mouse models.

摘要

MAIT 细胞是表达半不变αβ TCR 的非常规 T 细胞，它们通过高度保守的 MR1 蛋白识别细菌代谢产物。MAIT 细胞与肠道微生物群相互作用，文献报道 1 型糖尿病 (T1D) 中肠道内稳态的改变，提示 MAIT 细胞参与 T1D。由于 NOD 小鼠是 T1D 的成熟小鼠模型，因此在这些小鼠中研究了 MAIT 细胞，以评估它们在疾病发展中的潜在作用。本章描述了表征 MAIT 细胞并确定其在 T1D 小鼠模型中功能所需的材料和方法。