编码含malectin样结构域（MLD）和富含亮氨酸重复序列（LRR）蛋白的基因在……中的表达分析
Expression analysis of genes encoding malectin-like domain (MLD)- and leucine-rich repeat (LRR)- containing proteins in .
作者信息
Sultana Mst Momtaz, Hachiya Takushi, Dutta Amit Kumar, Nishimura Kohji, Suzuki Takamasa, Tanaka Ai, Nakagawa Tsuyoshi
机构信息
Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, Japan.
Department of Agricultural Extension (DAE), Ministry of Agriculture, Dhaka, Bangladesh.
出版信息
Biosci Biotechnol Biochem. 2020 Jan;84(1):154-158. doi: 10.1080/09168451.2019.1661769. Epub 2019 Sep 5.
Malectin is a maltose-binding endoplasmic reticulum protein conserved in animals. In , we identified four genes that encode malectin-like domain (MLD)- and leucine-rich repeat (LRR)-containing proteins (AtMLLRs): two were receptor-like proteins (AtMLLR1 and 2) and the other two were extracellular proteins (AtMLLR3 and 4). The promoter:G3GFP+promoter:GUS assay indicated the organ- and cell-specific expression of the and genes. Cm: chloramphenicol-resistance marker; G3GFP: G3 green fluorescent protein; GUS: -glucuronidase; KD: kinase domain; LRR: leucine-rich repeat; MLD: malectin-like domain; RLK: receptor-like kinase; SP: signal peptide; TMD: transmembrane domain; Tnos: nopaline synthase terminator.
Malectin是一种在动物中保守的结合麦芽糖的内质网蛋白。在本研究中，我们鉴定了四个编码含有malectin样结构域（MLD）和富含亮氨酸重复序列（LRR）的蛋白（AtMLLR）的基因：两个是类受体蛋白（AtMLLR1和2），另外两个是细胞外蛋白（AtMLLR3和4）。启动子：G3GFP +启动子：GUS分析表明了这些基因在器官和细胞中的特异性表达。Cm：氯霉素抗性标记；G3GFP：G3绿色荧光蛋白；GUS：β-葡萄糖醛酸酶；KD：激酶结构域；LRR：富含亮氨酸重复序列；MLD：malectin样结构域；RLK：类受体激酶；SP：信号肽；TMD：跨膜结构域；Tnos：胭脂碱合酶终止子。
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