Hossain Md Firose, Sultana Mst Momtaz, Tanaka Ai, Dutta Amit Kumar, Hachiya Takushi, Nakagawa Tsuyoshi
Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan.
Bioresource and Life Sciences, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, Japan.
Biochem Biophys Rep. 2022 Mar 5;30:101241. doi: 10.1016/j.bbrep.2022.101241. eCollection 2022 Jul.
contains a family of nine genes known as plant intracellular Ras-group related leucine-rich repeat (LRR) proteins (PIRLs). These are structurally similar to animals and fungal LRR proteins and play important roles in developmental pathways. However, to date, no detailed tissue-specific expression analysis of these has been performed. Therefore, in this study, we generated promoter:GUS transgenic plants for the nine genes and identified their expression patterns in seedlings and floral organs at different developmental stages. Most members showed expression in the root apical region and in the vascular tissue of primary and lateral roots. Shoot apex-specific expression was recorded for and . Furthermore, , , and showed distinct expression patterns in flowers, especially in pollen and anthers. In addition, co-expression network analysis identified cases where were co-expressed with other genes known to have specific functions related to growth and development. Taken together, the tissue-specific expression patterns of genes improve our understanding of the functions of this gene family in plant growth and development.
包含一个由九个基因组成的家族，称为植物细胞内Ras族相关富含亮氨酸重复序列（LRR）蛋白（PIRLs）。这些蛋白在结构上与动物和真菌的LRR蛋白相似，并在发育途径中发挥重要作用。然而，迄今为止，尚未对这些基因进行详细的组织特异性表达分析。因此，在本研究中，我们构建了这九个基因的启动子：GUS转基因植物，并确定了它们在不同发育阶段的幼苗和花器官中的表达模式。大多数PIRL成员在根尖区域以及主根和侧根的维管组织中表达。记录到PIRL1和PIRL5在茎尖特异性表达。此外，PIRL3、PIRL4、PIRL6和PIRL7在花中表现出不同的表达模式，尤其是在花粉和花药中。此外，共表达网络分析确定了PIRLs与其他已知具有与生长和发育相关特定功能的基因共表达的情况。综上所述，PIRL基因的组织特异性表达模式提高了我们对该基因家族在植物生长和发育中功能的理解。
