体外胃肠消化后水稻花青苷- Hohenbuehelia serotina 多糖复合物的抗增殖活性。
Anti-proliferation activities of Oryza sativa L. anthocyanins-Hohenbuehelia serotina polysaccharides complex after in vitro gastrointestinal digestion.
机构信息
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China.
出版信息
Food Chem Toxicol. 2020 Jan;135:111012. doi: 10.1016/j.fct.2019.111012. Epub 2019 Nov 30.
In order to improve the bioavailability of Oryza sativa L. anthocyanins, we fabricated Oryza sativa L. anthocyanins-Hohenbuehelia serotina polysaccharides (OSA-HSP) complex and investigated its anti-proliferation activities taking into account its changes along simulated gastrointestinal digestion in vitro. Results showed that OSA mainly composed by delphinidin, cyanidin, petunidin, malvidin and their derivatives was combined with HSP through electrostatic interaction. OSA-HSP complex belonged to non-crystalline substance, and had compact and laminar structural characteristics. Under simulated gastrointestinal digestion, OSA-HSP complex significantly prevented the degradation of anthocyanins, and presented sustain release effect. However, the anti-proliferation activities of OSA-HSP complex digested by different gastrointestinal process were remarkably changed, especially after small intestinal digestion. HeLa cells treated with OSA-HSP complex exhibited pro-apoptosis characteristics by triggering endogenous mitochondrial apoptosis pathway through activating the expressions of Bax, cytochrome c and Caspase-3 as well as inhibiting the expression of Bcl-2. These findings provided new insight to improve the bioavailability of anthocyanins in functional foods and tumor therapy.
为提高稻花素的生物利用度，我们制备了稻花素- Hohenbuehelia serotina 多糖（OSA-HSP）复合物，并考虑其在体外模拟胃肠道消化过程中的变化，研究了其抗增殖活性。结果表明，OSA 主要由矢车菊素、花青素、芍药素、锦葵素及其衍生物组成，通过静电相互作用与 HSP 结合。OSA-HSP 复合物属于无定形物质，具有紧密层状的结构特征。在模拟胃肠道消化过程中，OSA-HSP 复合物能显著防止花色苷的降解，并呈现出持续释放的效果。然而，经过不同胃肠道过程消化后的 OSA-HSP 复合物的抗增殖活性发生了显著变化，尤其是在小肠消化后。OSA-HSP 复合物处理的 HeLa 细胞通过激活 Bax、细胞色素 c 和 Caspase-3 的表达以及抑制 Bcl-2 的表达，触发内源性线粒体凋亡途径，表现出促凋亡的特征。这些发现为提高功能性食品和肿瘤治疗中花色苷的生物利用度提供了新的思路。
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