Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China; Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, PR China.
Food Chem Toxicol. 2021 Apr;150:112100. doi: 10.1016/j.fct.2021.112100. Epub 2021 Mar 4.
The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP). Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 10 to 4.55 × 10 Da. JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics. Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice. Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP. These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.
本研究旨在探讨模拟胃肠道消化对核桃壳多糖（JRP）结构特征和抗增殖活性的影响。结果表明，JRP 由葡萄糖、核糖、半乳糖、甘露糖、阿拉伯糖和鼠李糖组成，摩尔比为 10.7:4.9:16.4:2.3:10.8:2.3，分子量分布在 3.21×10 到 4.55×10 Da 之间。JRP 属于非晶态物质，具有不规则、光滑和紧凑的形态特征。然而，在体外胃肠道消化过程中，JRP 的理化性质，包括分子量、单糖组成、结晶性质和形态，都发生了显著变化，同时消化液中还原糖的含量也增加了。通过抗增殖活性的测定，结果表明，消化后的 JRP 通过诱导细胞凋亡，显著抑制 HeLa 细胞的活力，这是由于过量 ROS 积累和细胞周期停滞在 G2/M 期。所有这些都明显强于未消化的 JRP 诱导的结果。这些发现表明，经过胃肠道消化处理的 JRP 具有更强的潜在抗增殖应用潜力，值得进一步开发。
