Department of Microbiology and Mycology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10719 Olsztyn, Poland.
Chemistry Department, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Biomolecules. 2019 Nov 28;9(12):796. doi: 10.3390/biom9120796.
Pseudomonas KT2440, one of the best characterized pseudomonads, is a metabolically versatile producer of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) that serves as a model bacterium for molecular studies. The synthesis of mcl-PHAs is of great interest due to their commercial potential. Carbon and phosphorus are the essential nutrients for growth and their limitation can trigger mcl-PHAs' production in microorganisms. However, the specific molecular mechanisms that drive this synthesis in species under unfavorable growth conditions remain poorly understood. Therefore, the proteomic responses of KT2440 to the limited carbon and phosphorus levels in the different growth phases during mcl-PHAs synthesis were investigated. The data indicated that biopolymers' production was associated with the cell growth of KT2440 under carbon- and phosphorus-limiting conditions. The protein expression pattern changed during mcl-PHAs synthesis and accumulation, and during the different physiological states of the microorganism. The data suggested that the majority of metabolic activities ceased under carbon and phosphorus limitation. The abundance of polyhydroxyalkanoate granule-associated protein (PhaF) involved in PHA synthesis increased significantly at 24 and 48 h of the cultivations. The activation of proteins belonging to the phosphate regulon was also detected. Moreover, these results indicated changes in the protein profiles related to amino acids metabolism, replication, transcription, translation, stress response mechanisms, transport or signal transduction. The presented data allowed the investigation of time-course proteome alterations in response to carbon and phosphorus limitation, and PHAs synthesis. This study provided information about proteins that can be potential targets in improving the efficiency of mcl-PHAs synthesis.
铜绿假单胞菌 KT2440 是一种代谢能力很强的中链长度聚羟基烷酸（mcl-PHA）生产菌，是分子研究的模式细菌。由于其商业潜力，mcl-PHA 的合成受到了极大的关注。碳和磷是微生物生长所必需的营养物质，其限制可以触发微生物中 mcl-PHA 的生产。然而，在不利的生长条件下， 物种中驱动这种合成的具体分子机制仍知之甚少。因此，研究了 KT2440 在 mcl-PHAs 合成不同生长阶段的有限碳和磷水平下的蛋白质组响应。数据表明，在碳和磷限制条件下，生物聚合物的生产与 KT2440 的细胞生长有关。在 mcl-PHAs 合成和积累过程中以及在微生物的不同生理状态下，蛋白质表达模式发生了变化。数据表明，在碳和磷限制下，大多数代谢活动停止。与 PHA 合成有关的多聚羟基烷酸颗粒相关蛋白（PhaF）的丰度在培养 24 和 48 小时时显著增加。还检测到属于磷酸盐调节子的蛋白质的激活。此外，这些结果表明与氨基酸代谢、复制、转录、翻译、应激反应机制、运输或信号转导相关的蛋白质图谱发生了变化。所呈现的数据允许研究响应碳和磷限制以及 PHAs 合成的时间过程蛋白质组变化。这项研究提供了有关可以作为提高 mcl-PHA 合成效率的潜在目标的蛋白质的信息。
