Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan.
Department of Environmental Science, Islamic International University Islamabad, Islamabad, Pakistan.
Methods Mol Biol. 2020;2094:49-58. doi: 10.1007/978-1-0716-0183-9_6.
Rhizobacteria are known to produce a variety of signal molecules which may modify plant growth by interfering with phytohormone balance. Among the microbial signals are phytohormones, known to contribute to plant endogenous pool of phytohormones. The current chapter describes different methods to study the regulation of gene expression in root apical meristem in response to rhizobacterial inoculation. We describe protocol for the detection of in planta modulation of CKs and IAA by rhizobacteria and their impact on root growth, dissecting the underlying plant signaling pathway by RNA sequencing.
根际细菌会产生各种信号分子，通过干扰植物激素平衡来调节植物生长。其中的微生物信号包括植物激素，它们被认为是植物内源激素库的贡献者。本章描述了不同的方法来研究根冠细胞对根际细菌接种的反应中基因表达的调控。我们描述了通过根际细菌检测 CK 和 IAA 在植物体内的调制及其对根生长的影响的方案，通过 RNA 测序剖析潜在的植物信号通路。
