Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey.
Methods Mol Biol. 2020;2094:137-148. doi: 10.1007/978-1-0716-0183-9_15.
Plant pathogens cause different diseases on crops and industrial plant species that result in economic losses. Pathogen-free plant material has usually been obtained by traditional procedures such as meristem culture, thermotherapy, and chemotherapy. However, there are many limitations of these procedures such as mechanical challenges of meristem excision and low regeneration rate, low resistance to high temperatures, phytotoxicity, and mutagenic effects of the chemicals used in the procedures. Cryotherapy is a newly developed biotechnological tool that has been very effective in virus elimination from economically important plant species. This tool has overcome the abovementioned limitations. This chapter aims to highlight the importance of the cryogenic procedures (vitrification, encapsulation-vitrification, droplet vitrification, two-step freezing, dehydration, encapsulation-dehydration) in order to generate virus-free germplasm.
植物病原体可导致农作物和工业植物物种发生不同的疾病，从而造成经济损失。无病原体的植物材料通常通过传统程序获得，例如茎尖培养、热疗和化学疗法。然而，这些程序存在许多局限性，例如茎尖切除的机械挑战和低再生率、对高温的低抗性、化学物质的植物毒性和诱变作用。低温疗法是一种新开发的生物技术工具，已在从经济上重要的植物物种中消除病毒方面非常有效。该工具克服了上述限制。本章旨在强调低温程序（玻璃化、包埋-玻璃化、液滴玻璃化、两步冷冻、脱水、包埋-脱水）在产生无病毒种质方面的重要性。
