School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China.
Eur Rev Med Pharmacol Sci. 2019 Nov;23(22):9900-9906. doi: 10.26355/eurrev_201911_19555.
To explore the relationship between AK5 and gastric cancer.
The in situ levels of AK5 in the GC tissues from 255 patients were detected by immunohistochemistry (IHC). The correlation between AK5 expression and the clinicopathological parameters was analyzed by Pearson correlation, and the prognostic factors were identified by Cox regression analysis. The transcriptome data of 14 human GC cell lines deposited in the CCLE database were analyzed, and two lines were selected for functional studies. AK5 was knocked down in the AZ521 and MKN74 cells using siRNA, and their proliferation and apoptosis were evaluated by Cell Counting Kit-8 (CCK-8) assay and Annexin-V staining, respectively. In addition, the apoptosis and autophagy of the markers were detected by Western blotting.
Patients expressing high AK5 levels in the tumor tissues had significantly shorter survival compared to low-expressing group. In addition, AK5 expression was associated with T stage and N stage and was an independent prognostic factor. AK5 knockdown in the AZ521 and MKN74 cells significantly inhibited proliferation and autophagy, and increased apoptosis.
AK5 is a potential prognostic marker and therapeutic target for GC.
探索 AK5 与胃癌的关系。
采用免疫组织化学（IHC）检测 255 例胃癌患者 GC 组织中 AK5 的原位水平。通过 Pearson 相关性分析，分析 AK5 表达与临床病理参数之间的相关性，采用 Cox 回归分析确定预后因素。分析 CCLE 数据库中 14 个人类 GC 细胞系的转录组数据，选择两条进行功能研究。采用 siRNA 敲低 AZ521 和 MKN74 细胞中的 AK5，通过细胞计数试剂盒（CCK-8）检测和 Annexin-V 染色分别评估细胞增殖和凋亡。此外，通过 Western blot 检测凋亡和自噬标志物。
肿瘤组织中 AK5 高表达的患者与低表达组相比，生存时间明显缩短。此外，AK5 表达与 T 分期和 N 分期相关，是独立的预后因素。AK5 在 AZ521 和 MKN74 细胞中的敲低显著抑制了增殖和自噬，增加了凋亡。
AK5 是 GC 的一个潜在的预后标志物和治疗靶点。
