Uenoyama Kei, Ueno Yuichi, Tosaki Kaori, Abeto Yusuke, Ito Hirotaka, Katsuda Ken, Shibahara Tomoyuki
Pathological and Physiochemical Examination Division, Laboratory Department, Animal Quarantine Service, 11-1 Haramachi, Isogo, Yokohama, Kanagawa 235-0008, Japan.
Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2020 Jan 17;82(1):89-93. doi: 10.1292/jvms.19-0561. Epub 2019 Dec 4.
A 1-month-old rabbit, imported as a pet by a distributor, died suddenly in the quarantine period in Japan due to suppurative pleuropneumonia. A bacterial isolate from its right lung was identified as Pasteurella multocida serotype A: 11. The isolate was classified as ST204 using the RIRDC scheme of multilocus sequence typing, suggesting that the isolate was genetically related to European isolates of the same sequence type listed in the PubMLST database and not to four other isolates that originated from past imported rabbits. In the immunohistochemical assay, an antiserum recognizing the somatic serotype 11 antigen generated from chicken could specifically detect P. multocida, indicating that the antiserum for somatic serotyping was useful for immunohistochemical diagnosis of rabbit pasteurellosis.
一只由经销商作为宠物进口的1月龄兔子，在日本隔离期间因化脓性胸膜肺炎突然死亡。从其右肺分离出的一株细菌被鉴定为多杀性巴氏杆菌A:11血清型。使用多位点序列分型的RIRDC方案将该分离株分类为ST204，这表明该分离株在基因上与PubMLST数据库中列出的相同序列类型的欧洲分离株相关，而与过去进口兔子的其他四个分离株无关。在免疫组织化学检测中，一种识别由鸡产生的体细胞血清型11抗原的抗血清可以特异性检测多杀性巴氏杆菌，这表明用于体细胞血清分型的抗血清可用于兔巴氏杆菌病的免疫组织化学诊断。
