Department Pharmaceutical Microbiology, Hans-Knöll-Institute, Friedrich-Schiller-Universität, Beutenbergstrasse 11a, 07745, Jena, Germany.
Chembiochem. 2020 May 4;21(9):1364-1371. doi: 10.1002/cbic.201900649. Epub 2020 Jan 10.
Psychotropic Psilocybe mushrooms biosynthesize their principal natural product psilocybin in five steps, among them a phosphotransfer and two methyltransfer reactions, which consume one equivalent of 5'-adenosine triphosphate (ATP) and two equivalents of S-adenosyl-l-methionine (SAM). This short but co-substrate-intensive pathway requires nucleoside cofactor salvage to maintain high psilocybin production rates. We characterized the adenosine kinase (AdoK) and S-adenosyl-l-homocysteine (SAH) hydrolase (SahH) of Psilocybe cubensis. Both enzymes are directly or indirectly involved in regenerating SAM. qRT-PCR expression analysis revealed an induced expression of the genes in the fungal primordia and carpophores. A one-pot in vitro reaction with the N-methyltransferase PsiM of the psilocybin pathway demonstrates a concerted action with SahH to facilitate biosynthesis by removal of accumulating SAH.
迷幻蘑菇灵芝属在五个步骤中生物合成其主要天然产物裸盖菇素，其中包括一个磷酸转移和两个甲基转移反应，消耗一个 5'-三磷酸腺苷（ATP）和两个 S-腺苷甲硫氨酸（SAM）的等价物。这个简短但共底物密集的途径需要核苷辅酶补救来维持高裸盖菇素生产速率。我们对迷幻蘑菇灵芝属的腺苷激酶（AdoK）和 S-腺苷同型半胱氨酸（SAH）水解酶（SahH）进行了表征。这两种酶都直接或间接地参与了 SAM 的再生。qRT-PCR 表达分析显示，真菌原基和子实体中基因的表达被诱导。与裸盖菇素途径的 N-甲基转移酶 PsiM 进行的一锅体外反应表明，与 SahH 协同作用，通过去除积累的 SAH 来促进生物合成。
