College of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang, 453003, China.
Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Mikrochim Acta. 2019 Dec 5;187(1):18. doi: 10.1007/s00604-019-4004-6.
A colorimetric method is described for the determination of uric acid (UA). The assay is based on oxidative etching of silver nanoparticles (AgNPs) by chloroauric acid (HAuCl). The presence of UA suppresses the redox reaction between AgNPs and HAuCl because a competitive reaction occurs between HAuCl and UA. This results in a color change of the solution from brown to yellow. In parallel, the absorbance is blue shifted from 477 to 428 nm. The method has a detection limit as low as 30 pM (at S/N = 3) and a linear response range that covers the 0.1 nM to 0.1 mM UA concentration range. The reliability of the method was successfully demonstrated by analyzing spiked serum samples. Graphical abstractSchematic representation of a colorimetric method for determination of uric acid (UA) based on oxidative etching of silver nanoparticles by HAuCl.
描述了一种用于测定尿酸 (UA) 的比色法。该测定法基于氯金酸 (HAuCl) 对银纳米粒子 (AgNPs) 的氧化刻蚀。UA 的存在抑制了 AgNPs 和 HAuCl 之间的氧化还原反应，因为 HAuCl 和 UA 之间发生了竞争性反应。这导致溶液的颜色从棕色变为黄色。同时，吸光度从 477nm 蓝移至 428nm。该方法的检测限低至 30pM（信噪比为 3），线性响应范围涵盖 0.1nM 至 0.1mM 的 UA 浓度范围。通过分析加标血清样品成功证明了该方法的可靠性。
