Department of Chemistry, Nanchang University, Nanchang, 330031, Jiangxi, China.
The Fourth Affiliated Hospital, Nanchang University, Nanchang, 330003, Jiangxi, China.
Anal Bioanal Chem. 2019 Feb;411(4):943-952. doi: 10.1007/s00216-018-1524-6. Epub 2018 Dec 12.
A simple, rapid, and effective colorimetric assay for the detection of uric acid (UA) has been built, using the MoS nanoflakes-catalyzed 3,3',5,5'-tetramethylbenzidine (TMB)-HO system. In the presence of oxygen and uricase, uric acid was oxidized specifically to produce HO. MoS nanoflakes synthesized by hydrothermal reaction could catalyze the oxidation of TMB by HO, and engendering the colorimetric signal. Finally, the change in the color from colorless to blue was seen with naked eye, indicating the presence or absence of UA. Under the optimized conditions, a linear relationship between the absorbance and UA concentration in the range of 0.5-100 μM (R = 0.996) with the limit of detection for 0.3 μM (S/N = 3). The proposed assay was successfully applied to the detection of UA in human serum with the recoveries over 94.54%. Thus, these results suggest that the UA assay-based MoS-catalyzed TMB-HO has great foreground for fast clinical diagnosis of gout without the need for advanced and costly equipment. Graphical Abstract ᅟ.
一种简单、快速、有效的尿酸（UA）比色检测方法已经建立，使用的是 MoS 纳米片催化的 3,3',5,5'-四甲基联苯胺（TMB）-HO 体系。在氧气和尿酸酶存在的情况下，尿酸被特异性氧化生成 HO。通过水热反应合成的 MoS 纳米片可以催化 HO 氧化 TMB，从而产生比色信号。最后，肉眼可以看到颜色从无色变为蓝色，表明是否存在 UA。在优化条件下，吸光度与 0.5-100 μM 范围内 UA 浓度呈线性关系（R=0.996），检测限为 0.3 μM（S/N=3）。该方法成功应用于人血清中 UA 的检测，回收率超过 94.54%。因此，这些结果表明，基于 MoS 催化 TMB-HO 的 UA 测定法具有无需先进和昂贵设备即可快速临床诊断痛风的巨大前景。
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