Hunan University of Chinese Medicine, Changsha, Hunan Province, China.
Hunan Institute of Nuclear Agricultural Sciences and Space-induced Breeding, Changsha, Hunan province, China.
PLoS One. 2019 Dec 6;14(12):e0225802. doi: 10.1371/journal.pone.0225802. eCollection 2019.
The current study aimed to investigate the effects of Debaryomyces hansenii on the diversity of bacterial lactase gene in the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice.
Eighteen mice were randomly divided into three groups (6 mice per group): healthy control group, diarrhea model group and D. hansenii treatment group. The antibiotic-associated diarrhea model was established by intragastric administration with a mixture of cephradine and gentamicin sulfate (23.33 mL·kg-1·d-1) twice a day for 5 days continuously. After establishing the AAD model, the mice in the D. hansenii treatment group were gavaged with D. hansenii for three days, while other groups were gavaged with distilled water. Then, the intestinal mucosa of all three groups was collected and DNA was extracted in an aseptic environment for the following analysis.
The difference in the richness and homogeneity of the bacterial lactase gene among all samples were inapparent, as the difference in the Chao1, ACE, Simpson and Shannon indices among the three groups were insignificant (P>0.05). NMDS analysis also showed that the distance of the samples among the three groups was unobvious. Furthermore, the bacterial lactase gene in the mucosa mainly originated from Actinobacteria, Firmicutes and Proteobacteria. Compared with the healthy control group, the abundance of lactase genes originating from Cupriavidus, Lysobacter, Citrobacter, Enterobacter and Pseudomonas was increased in the D. hansenii treatment group, while the lactase gene from Acidovorax and Stenotrophomonas decreased (p < 0.01 or p < 0.05) in the diarrhea model group and the D. hansenii treatment group.
D. hansenii was capable of improving the growth of some key lactase-producing bacteria like Deinococcus, Cupriavidus and Lysobacter for treating AAD.
本研究旨在探讨德巴利酵母对抗生素相关性腹泻（AAD）小鼠肠黏膜细菌乳糖酶基因多样性的影响。
将 18 只小鼠随机分为 3 组（每组 6 只）：健康对照组、腹泻模型组和德巴利酵母治疗组。采用头孢拉定和硫酸庆大霉素（23.33 mL·kg-1·d-1）混合液连续 2 天每天 2 次灌胃建立 AAD 模型。建立 AAD 模型后，德巴利酵母治疗组小鼠连续 3 天灌胃德巴利酵母，其他组灌胃蒸馏水。然后在无菌环境下采集所有 3 组的肠黏膜，提取 DNA，进行以下分析。
所有样本细菌乳糖酶基因的丰富度和均匀度差异不明显，3 组的 Chao1、ACE、Simpson 和 Shannon 指数差异无统计学意义（P>0.05）。NMDS 分析也表明 3 组样本之间的距离不明显。此外，黏膜中的细菌乳糖酶基因主要来源于放线菌、厚壁菌门和变形菌门。与健康对照组相比，德巴利酵母治疗组中来源于 Cupriavidus、Lysobacter、Citrobacter、Enterobacter 和 Pseudomonas 的乳糖酶基因丰度增加，而腹泻模型组和德巴利酵母治疗组中来源于 Acidovorax 和 Stenotrophomonas 的乳糖酶基因丰度降低（p<0.01 或 p<0.05）。
德巴利酵母能够改善德氏乳杆菌、铜绿假单胞菌和赖氨酸芽孢杆菌等一些关键产乳糖酶细菌的生长，从而治疗 AAD。
