MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China.
Genomics. 2020 Mar;112(2):2080-2091. doi: 10.1016/j.ygeno.2019.12.002. Epub 2019 Dec 3.
Manganese (Mn) nodule is one of the ubiquitous polymetallic concretions and mainly consists of Mn - Fe oxi-hydroxide precipitations. A primary oxidation of Mn(II) to MnO, in which microorganisms may play important roles, is followed by agglomeration of MnO into nodules. Celeribater manganoxidans DY25, belonging to family Rhodobacteraceae, has ability to catalyze the formation of MnO [1]. The concentration of MnO formed by harvested cells reached 7.08 μM after suspended in 10 mM HEPES (pH 7.5). Genomic and physiological characteristics of strain DY25 provided a better understanding of its Mn-oxidizing mechanism. Fifteen genes (including four multicopper oxidases) may be involved in Mn(II)-oxidation, whereas only three of them can promote this process. Sulfur-oxidizing activity was detected, which may be associated with manganese oxidation. Genes involved in import and export of primary elemental ingredients (C, N, P and S) and metallic elements (e.g. Mn) were discovered, demonstrating its potential roles in the biogeochemical cycle.
锰结核是一种普遍存在的多金属结核，主要由 Mn-Fe 氧化氢沉淀物组成。Mn(II)的初步氧化为 MnO，微生物可能在此过程中发挥重要作用，随后 MnO 聚集成结核。属于红杆菌科的 Celeribacter manganoxidans DY25 具有催化 MnO 形成的能力[1]。将收获的细胞悬浮在 10 mM HEPES（pH 7.5）中后，形成的 MnO 浓度达到 7.08 μM。DY25 菌株的基因组和生理特性使我们更好地了解了其 Mn 氧化机制。有 15 个基因（包括四个多铜氧化酶）可能参与 Mn(II)氧化，而只有其中三个基因可以促进这一过程。检测到硫氧化活性，这可能与锰氧化有关。发现了涉及主要元素（C、N、P 和 S）和金属元素（如 Mn）的输入和输出的基因，表明其在生物地球化学循环中具有潜在作用。
