Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA.
Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA.
Int J Pharm. 2020 Feb 25;576:118903. doi: 10.1016/j.ijpharm.2019.118903. Epub 2019 Dec 3.
The stratum corneum (SC), the outermost layer of the skin and its major barrier for penetration, contains furrows of different depths on its surface. The presence of these furrows might lead to erroneous interpretation of the results in skin permeation studies using tape stripping, in which the material trapped in the furrows removed by the tapes representing different layers of the SC might be interpreted as material penetrating within these layers. The present objective was to investigate the effect of skin furrows on tape stripping results. Non-penetrating fluorescent materials were topically applied to split-thickness human and full-thickness porcine skin samples. Tape stripping was applied, and the tapes were assessed by fluorescence microscopy and quantitative analyses. The microscopy images were assessed visually to determine the presence of the applied material in the furrows. The penetration depth of the material was examined and the fluorescence content and pattern in each tape were analyzed. The results suggested that skin furrows could be important in the first 10 tapes, affecting the quantification of materials in the SC, particularly in permeation studies of materials with low penetration into the SC. Depending on the properties of the materials, skin rinsing could reduce the impact of furrows.
角质层（SC）是皮肤的最外层，也是渗透的主要屏障，其表面存在不同深度的凹槽。这些凹槽的存在可能导致在使用胶带剥离进行皮肤渗透研究时对结果产生错误的解释，因为胶带去除的被困在凹槽中的物质可能被解释为穿透这些层的物质。本研究旨在探讨皮肤凹槽对胶带剥离结果的影响。非渗透荧光物质被局部应用于人体和全层猪皮样本。进行胶带剥离，并通过荧光显微镜和定量分析评估胶带。通过肉眼评估显微镜图像以确定凹槽中是否存在应用材料。检查了材料的渗透深度，并分析了每个胶带中的荧光含量和模式。结果表明，在最初的 10 个胶带中，皮肤凹槽可能很重要，会影响 SC 中物质的定量分析，尤其是在低渗透 SC 的物质渗透研究中。根据材料的性质，皮肤冲洗可以减少凹槽的影响。
