Bashir S J, Chew A L, Anigbogu A, Dreher F, Maibach H I
Department of Dermatology, University of California, School of Medicine, San Francisco, CA, USA.
Skin Res Technol. 2001 Feb;7(1):40-8. doi: 10.1034/j.1600-0846.2001.007001040.x.
BACKGROUND/AIMS: Tape stripping of human stratum corneum has been performed to measure stratum corneum mass, barrier function, drug reservoir and percutaneous penetration. However, the technique itself requires further development to facilitate interpretation.
In this study we quantified stratum comeum (SC) tape stripping and water kinetic parameters utilizing three types of adhesive tapes, in an in vivo randomized clinical trial. Stratum corneum was tape stripped, and the mass of SC removed by each tape was quantified utilizing a protein assay. Transepidermal water loss (TEWL) was measured and barrier disruption and SC water kinetics calculated. Three commonly utilized acrylate adhesive tapes were utilized and a comparison made between them.
Each type of tape successfully stripped the stratum corneum, but the rayon tape did not induce SC barrier disruption. Neither the type of tape nor the site stripped significantly influenced the mass of SC removed. Water kinetic parameters did not differ significantly for the tapes that did induce barrier disruption. Individual variation in barrier disruption to water following tape stripping was demonstrated.
The tapes utilized removed a similar amount of SC. The tapes have a different propensity to cause barrier disruption. Some individuals do not demonstrate increased TEWL despite an equivalent mass of SC being removed compared to those who do show a response.
背景/目的：已采用胶带剥离人体角质层来测量角质层质量、屏障功能、药物储存库及经皮渗透。然而，该技术本身需要进一步改进以利于解读。
在本体内随机临床试验中，我们使用三种类型的胶带对角质层胶带剥离和水动力学参数进行了量化。对角质层进行胶带剥离，利用蛋白质测定法对每条胶带去除的角质层质量进行量化。测量经表皮水分流失（TEWL），并计算屏障破坏和角质层水动力学。使用了三种常用的丙烯酸酯胶带并对它们进行比较。
每种胶带均成功剥离角质层，但人造丝胶带未引起角质层屏障破坏。胶带类型和剥离部位均未显著影响去除的角质层质量。对于确实引起屏障破坏的胶带，水动力学参数无显著差异。证明了胶带剥离后屏障对水破坏的个体差异。
所使用的胶带去除的角质层量相似。这些胶带导致屏障破坏的倾向不同。与那些有反应的人相比，一些人尽管去除的角质层质量相当，但并未表现出TEWL增加。
