In-situ detection of cadmium with aptamer functionalized gold nanoparticles based on smartphone-based colorimetric system.

Cadmium is a heavy metal pollutant in environment with high toxicity that severely threats human health. A simple and sensitive method for rapid detection of cadmium ions in water sample is of significant importance. In this paper, a colorimetric method based on aptamer-functionalized gold nanoparticles (AuNPs) for specific recognition were proposed to realize Cd detection. AuNPs aggregate in high-salt solutions because of the shielding of salt to electrostatic repulsion among AuNPs, while aptamers can strengthen the stability of AuNPs and avoid the aggregation. After adding Cd ions, the specific interaction between aptamers and Cd leads to a decrease of free aptamers, which weakens the stability of the AuNPs and results in the color change of the solution. The colorimetric change can be rapidly captured and analyzed by a self-developed smartphone-based colorimetric system (SBCS) within 10 min, which implements the quantitative detection of Cd. The results show that Cd ions can be detected with high selectivity and sensitivity with a linear range of 2-20 μg/L and a detection limit of 1.12 μg/L. Compared with other methods, the proposed approach features high sensitivity, high simplicity, easy implementation and high throughout, which provides a promising means for in-situ determination of Cd in practical applications.