Faculty of Pharmacy, Department of Physical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416, Gdansk, Poland.
Department of Molecular Enzymology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
Drug Deliv Transl Res. 2020 Apr;10(2):455-470. doi: 10.1007/s13346-019-00686-3.
We reported the new biphasic composites of calcium phosphate and mesoporous silica material (CaP@MSi) in the form of powders and pellets as a potential bone drug delivery system for doxycycline hydrochloride (DOX). The CaP@MSi powders were synthesized by cationic surfactant-templating method. The effects of 10, 20, and 30% CaP content in the CaP@MSi powders on the molecular surface structure, the cytotoxicity against osteoblast cells in vitro, and the mineralization potential in simulated body fluid were investigated. The CaP@MSi characterized by the highest mineralization potential (30% CaP content) were used for DOX adsorption and pelletization process. The CaP which precipitated in the CaP@MSi composites was characterized as calcium-deficient with the Ca:P molar ratio between 1.0 and 1.2. The cytotoxicity assays demonstrated that the CaP content in MSi increases osteoblasts viability indicating the CaP@MSi (30% CaP content) as the most biocompatible. The combination of CaP and MSi was an effective strategy to improve the mineralization potential of parent material. Upon immersion in simulated body fluid, the CaP of composite converted into the bone-like apatite. The obtained pellets preserved the mineralization potential of CaP@MSi and provided the prolonged 5-day DOX release. The obtained biphasic CaP@MSi composites seem to have an application potential as bone-specific drug delivery system.
我们报道了一种新型的磷酸钙和介孔硅材料（CaP@MSi）的双相复合材料，以粉末和颗粒的形式作为盐酸多西环素（DOX）的潜在骨药物传递系统。CaP@MSi 粉末通过阳离子表面活性剂模板法合成。研究了 CaP@MSi 粉末中 10%、20%和 30%CaP 含量对分子表面结构、体外成骨细胞细胞毒性和模拟体液中矿化潜力的影响。具有最高矿化潜力（30%CaP 含量）的 CaP@MSi 用于 DOX 吸附和造粒过程。CaP@MSi 复合材料中沉淀的 CaP 特征为缺钙，Ca:P 摩尔比在 1.0 到 1.2 之间。细胞毒性测定表明，MSi 中的 CaP 含量增加了成骨细胞的活力，表明 CaP@MSi（30%CaP 含量）具有最高的生物相容性。CaP 和 MSi 的结合是提高母体材料矿化潜力的有效策略。在模拟体液中浸泡时，复合材料中的 CaP 转化为类骨磷灰石。所得的颗粒保留了 CaP@MSi 的矿化潜力，并提供了长达 5 天的 DOX 释放。所得的双相 CaP@MSi 复合材料似乎具有作为骨特异性药物传递系统的应用潜力。
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