College of Public Health, Zhengzhou University, Zhengzhou, People's Republic of China.
College of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
Colloids Surf B Biointerfaces. 2020 Feb;186:110700. doi: 10.1016/j.colsurfb.2019.110700. Epub 2019 Dec 2.
Recently, fullerene (C60) has been widely used as a nano photosensitizer (PS) for tumor related photodynamic therapy (PDT). However, current PDT based on C60 is severely restricted by the visible light source (shallow tissue penetrating depth) and oxygen dependent (tumor hypoxia). Therefore, taking advantages of the surface plasmon resonance (SPR) effect of gold nanoparticles (GNPs) and "electronic sponge" property of C60, a C60 based hybrid nanostructured photosensitizer (C60@GNPs) with high light stability, near infrared light (NIR) excitation, and oxygen non-dependent properties was rational designed according to the mechanism of PDT. Compared with C60, after GNPs in-situ synthesis, the PDT mechanism of C60@GNPs changed from type II to type I, and the main product of PDT changed from singlet oxygen to hydroxyl radicals. Furthermore, C60@GNPs hybrid could efficiently generate hydroxyl radicals under NIR light excitation even in the hypoxia condition. These results suggest that C60@GNPs hybrid has a great potential for in vivo PDT applications.
最近，富勒烯（C60）已被广泛用作与肿瘤相关的光动力疗法（PDT）的纳米光敏剂（PS）。然而，目前基于 C60 的 PDT 受到可见光光源（组织穿透深度浅）和氧依赖性（肿瘤缺氧）的严重限制。因此，利用金纳米粒子（GNPs）的表面等离子体共振（SPR）效应和 C60 的“电子海绵”特性，根据 PDT 的机制，合理设计了具有高光稳定性、近红外光（NIR）激发和非氧依赖性特性的基于 C60 的杂化纳米结构光敏剂（C60@GNPs）。与 C60 相比，在原位合成 GNPs 后，C60@GNPs 的 PDT 机制从 II 型转变为 I 型，PDT 的主要产物从单线态氧变为羟基自由基。此外，即使在缺氧条件下，C60@GNPs 杂化也能在近红外光激发下有效产生羟基自由基。这些结果表明 C60@GNPs 杂化在体内 PDT 应用中有很大的潜力。
