Fukami H, Laing R A, Tsubota K, Chiba K, Oak S S
Department of Ophthalmology, Boston University School of Medicine, MA 02118.
Invest Ophthalmol Vis Sci. 1988 Nov;29(11):1677-82.
The healing processes that occur when corneal endothelial cells are subjected to only mild trauma are not known. To study these processes we have developed a system that enables only a few endothelial cells to be traumatized or destroyed under continuous specular microscopic observation in vitro. Experiments in which a small group of cells were traumatized by gentle wounding with a microglass tip produced an immediate and distinct dark area having the same size as the tip. Using this method we have produced and observed two types of wound by controlling the force of wounding. The first type of wound, produced by a gentle touch, recovered within 1 hr. The second type of wound, produced by moderate touch, took about 24 hr to recover completely from the trauma. In the second type of wound, we observed migration, elongation, coalescence and mitosis during the healing process. Histological examination revealed that in the first type of wound, the cells remained intact with no apparent damage seen by vital staining and light microscopy. For the second type of wound, the cells were completely missing although there was no apparent damage to Descemet's membrane.
当角膜内皮细胞仅受到轻度创伤时所发生的愈合过程尚不清楚。为了研究这些过程，我们开发了一种系统，该系统能够在体外连续镜面显微镜观察下仅使少数内皮细胞受到创伤或破坏。用微玻璃尖端轻轻损伤一小群细胞的实验产生了一个立即出现的、与尖端大小相同的明显暗区。使用这种方法，我们通过控制损伤力产生并观察了两种类型的伤口。第一种类型的伤口是由轻轻触碰产生的，在1小时内恢复。第二种类型的伤口是由适度触碰产生的，从创伤中完全恢复大约需要24小时。在第二种类型的伤口中，我们在愈合过程中观察到了迁移、伸长、融合和有丝分裂。组织学检查显示，在第一种类型的伤口中，细胞保持完整，活体染色和光学显微镜检查未发现明显损伤。对于第二种类型的伤口，细胞完全缺失，尽管后弹力层没有明显损伤。
