Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.
South Sichuan Institute of Translational Medicine, Luzhou, Sichuan, China.
BMC Genet. 2019 Dec 10;20(1):95. doi: 10.1186/s12863-019-0795-z.
Fish immunity is not only affected by the innate immune pathways but is also triggered by stress. Transport and loading stress can induce oxidative stress and further activate the immune inflammatory response, which cause tissue damage and sudden death. Multiple genes take part in this process and some of these genes play a vital role in regulation of the immune inflammatory response and sudden death. Currently, the key genes regulating the immune inflammatory response and the sudden death caused by stress in Coilia nasus are unknown.
In this study, we studied the effects of the Glo1 gene on stress, antioxidant expression, and immune-mediated apoptosis in C. nasus. The full-length gene is 4356 bp, containing six exons and five introns. Southern blotting indicated that Glo1 is a single-copy gene in the C. nasus genome. We found two single-nucleotide polymorphisms (SNPs) in the Glo1 coding region, which affect the three-dimensional structure of Glo1 protein. An association analysis results revealed that the two SNPs are associated with stress tolerance. Moreover, Glo1 mRNA and protein expression of the heterozygous genotype was significantly higher than that of the homozygous genotype. Na and sorbitol also significantly enhanced Glo1 mRNA and protein expression, improved the fish's antioxidant capacity, and reduced the immune inflammatory response, thus sharply reducing the mortality caused by stress.
Glo1 plays a potential role in the stress response, antioxidant capacity, and immune-mediated apoptosis in C. nasus.
鱼类的免疫不仅受先天免疫途径的影响，还受应激的影响。运输和装载应激会引起氧化应激，并进一步激活免疫炎症反应，导致组织损伤和突然死亡。多个基因参与了这一过程，其中一些基因在免疫炎症反应和应激引起的突然死亡的调节中起着至关重要的作用。目前，调控鲱鱼免疫炎症反应和应激引起的突然死亡的关键基因尚不清楚。
在本研究中，我们研究了 Glo1 基因对鲱鱼应激、抗氧化表达和免疫介导的细胞凋亡的影响。全长基因为 4356bp，包含六个外显子和五个内含子。Southern blot 分析表明，Glo1 是鲱鱼基因组中的单拷贝基因。我们在 Glo1 编码区发现了两个单核苷酸多态性（SNP），它们影响 Glo1 蛋白的三维结构。关联分析结果表明，这两个 SNP 与应激耐受性相关。此外，杂合基因型的 Glo1 mRNA 和蛋白表达明显高于纯合基因型。Na 和山梨醇也显著增强了 Glo1 mRNA 和蛋白表达，提高了鱼类的抗氧化能力，降低了免疫炎症反应，从而显著降低了应激引起的死亡率。
Glo1 在鲱鱼的应激反应、抗氧化能力和免疫介导的细胞凋亡中发挥了潜在的作用。
