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使用各种标记(³H、U-¹⁴C)葡萄糖示踪剂测定雏鸡(家鸡)的葡萄糖周转率。

Glucose turnover in the young chicken (Gallus domesticus) using variously labeled (3H, U-14C) glucose tracers.

作者信息

Brady L J, Romsos D R, Leveille G A

机构信息

Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824.

出版信息

Comp Biochem Physiol B. 1977;56(4):421-5. doi: 10.1016/0305-0491(77)90242-5.

DOI:10.1016/0305-0491(77)90242-5
PMID:318253
Abstract
  1. Various parameters of glucose metabolism--glucose replacement rate, percent recycling, mean transit time, and glucose mass were examined using various double labeled glucose tracers--2T-, U-14C; 3T-, U-14C; 4T-, U-14C; 5T-, U14C; and 6T-, U-14C. 2. Estimates of replacement rate were greatest for 2T-glucose (21.4 mg/min/kg), with 3T-, 4T-, 5T-, and 6T-glucose all having similar values (15.8, 15.6, 17.0, 16.0 mg/min/kg, respectively). 3. Calculated glucose mass based on all tritiated tracers (734-1086 mg/kg body weight) agreed closely with a direct determination of body glucose (969 mg/kg). 4. Reincorporation of tritium from 3H2O into glucose did not occur to any significant degree. 5. The young chick was found to have a very rapid rate of glucose turnover and high percent recycling compared to mammals.
摘要
  1. 使用各种双标记葡萄糖示踪剂——2T-、U-14C;3T-、U-14C;4T-、U-14C;5T-、U14C;以及6T-、U-14C,检测了葡萄糖代谢的各种参数——葡萄糖替代率、再循环百分比、平均转运时间和葡萄糖量。2. 2T-葡萄糖的替代率估计值最高(21.4毫克/分钟/千克),3T-、4T-、5T-和6T-葡萄糖的替代率值相似(分别为15.8、15.6、17.0、16.0毫克/分钟/千克)。3. 根据所有氚标记示踪剂计算出的葡萄糖量(734 - 1086毫克/千克体重)与直接测定的体内葡萄糖量(969毫克/千克)非常接近。4. 氚从3H2O重新掺入葡萄糖的程度不显著。5. 与哺乳动物相比,发现幼雏的葡萄糖周转速度非常快,再循环百分比很高。

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Glucose turnover in the young chicken (Gallus domesticus) using variously labeled (3H, U-14C) glucose tracers.使用各种标记(³H、U-¹⁴C)葡萄糖示踪剂测定雏鸡(家鸡)的葡萄糖周转率。
Comp Biochem Physiol B. 1977;56(4):421-5. doi: 10.1016/0305-0491(77)90242-5.
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Use of 3H and 14C doubly labeled glucose and amino acids in the study of hormonal regulation of gluconeogenesis in rats.利用3H和14C双标记葡萄糖和氨基酸研究大鼠糖异生的激素调节
Fed Proc. 1977 Feb;36(2):245-52.

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Mild type II diabetes markedly increases glucose cycling in the postabsorptive state and during glucose infusion irrespective of obesity.轻度II型糖尿病会显著增加空腹状态及葡萄糖输注期间的葡萄糖循环,且与肥胖无关。
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