Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, 100 Kexue Avenue, 450001, Henan, China.
Shaoxing Center of Diseases for Control and Prevention, Shaoxing, 312000, Zhejiang, China.
Eur J Clin Nutr. 2020 Jul;74(7):1091-1099. doi: 10.1038/s41430-019-0543-5. Epub 2019 Dec 11.
BACKGROUND/OBJECTIVES: The present cross-sectional study evaluated the association of vitamin D receptor (VDR) variants with serum 25(OH)D levels and their interaction on essential hypertension (EH) risk.
SUBJECTS/METHODS: 1539 patients were eligible in the study population. Two loci in VDR gene (rs2239179, rs2189480) were genotyped by TaqMan probe assays. Logistic regression, Kruskal-Wallis rank test and Chi-square test were used to determine the association among VDR polymorphisms, serum vitamin D metabolites, and the risk of EH. Interaction plots were performed to explain the interaction effects of circulating 25(OH)D levels and VDR variants on EH susceptibility.
After potential confounding adjustment, we observed that the mutations of VDR (rs2239179/rs2189480) were associated with the increased risk of EH (P < 0.05). Moreover, plasma 25(OH)D levels were inversely associated with EH, However, we did not find the association between serum 25(OH)D and VDR variants. When comparing with wild-type homozygous and heterozygous genotype carriers with vitamin D sufficiency, hypovitaminosis D and insufficient participants carrying homozygous variant genotype of rs2239179 showed a higher risk of EH, increased by 113% (OR = 2.13, 95% CI: 1.20, 3.80); Notably, the detrimental effect of rs2239179 homozygous variant on EH became stronger in the case of serum 25(OH)D <30 ng/ml. However, we did not find the interaction effect between rs2189480 variants and serum 25(OH)D levels on the risk of EH.
Our results suggested that the mutations of VDR may accelerate the progression of EH etiology, especially when suffering hypovitaminnosis D and insufficiency.
背景/目的：本横断面研究评估了维生素 D 受体（VDR）变体与血清 25（OH）D 水平的关联及其对原发性高血压（EH）风险的相互作用。
受试者/方法：研究人群中共有 1539 名符合条件的患者。使用 TaqMan 探针检测 VDR 基因中的两个位点（rs2239179、rs2189480）。使用逻辑回归、Kruskal-Wallis 秩检验和卡方检验来确定 VDR 多态性、血清维生素 D 代谢物与 EH 风险之间的关联。进行交互作用图以解释循环 25（OH）D 水平和 VDR 变体对 EH 易感性的相互作用效应。
在进行潜在混杂因素调整后，我们观察到 VDR（rs2239179/rs2189480）突变与 EH 风险增加相关（P<0.05）。此外，血浆 25（OH）D 水平与 EH 呈负相关。然而，我们没有发现血清 25（OH）D 与 VDR 变体之间的关联。与维生素 D 充足的野生型纯合子和杂合子基因型携带者相比，维生素 D 缺乏和不足的参与者携带 rs2239179 纯合变体基因型的患者发生 EH 的风险更高，增加了 113%（OR=2.13，95%CI：1.20，3.80）；值得注意的是，rs2239179 纯合变体对 EH 的不利影响在血清 25（OH）D<30ng/ml 的情况下更强。然而，我们没有发现 rs2189480 变体与血清 25（OH）D 水平对 EH 风险的交互作用。
我们的研究结果表明，VDR 的突变可能会加速 EH 病因的进展，尤其是在维生素 D 缺乏和不足的情况下。
