CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Archaea. 2019 Nov 18;2019:5108012. doi: 10.1155/2019/5108012. eCollection 2019.
Seagrass colonization alters sediment physicochemical properties by depositing seagrass fibers and releasing organic carbon and oxygen from the roots. How this seagrass colonization-induced spatial heterogeneity affects archaeal community structure and abundance remains unclear. In this study, we investigated archaeal abundance, diversity, and composition in both vegetated and adjacent bare surface sediments of a meadow. High-throughput sequencing of 16S rDNA showed that , , and were the most abundant phyla across all samples, accounting for approximately 42%, 21%, and 17% of the total archaeal communities, respectively. In terms of relative abundance, and were not significantly different between these two niches; however, specific subclades (Woese-3, Woese-21, Bathy-6, Bathy-18) were significantly enriched in vegetated sediments ( < 0.05), while was favored in unvegetated sites ( = 0.02). The quantification of archaeal 16S rRNA genes showed that the absolute abundance of the whole archaeal community, , and Woese-3, Woese-10, Woese-13, and Woese-21 was significantly more abundant in vegetated sediments than in bare sediments ( < 0.05). Our study expands the available knowledge of the distribution patterns and niche preferences of archaea in seagrass systems, especially for the different subclades of and , in terms of both relative proportions and absolute quantities.
海草定植通过沉积海草纤维和从根部释放有机碳和氧气来改变沉积物的物理化学性质。这种海草定植诱导的空间异质性如何影响古菌群落结构和丰度尚不清楚。在这项研究中，我们调查了草地中植被和相邻裸表面沉积物中的古菌丰度、多样性和组成。16S rDNA 的高通量测序表明，、和是所有样品中最丰富的门，分别约占总古菌群落的 42%、21%和 17%。就相对丰度而言，这两个生境之间的和没有显著差异；然而，特定的亚群（Woese-3、Woese-21、Bathy-6、Bathy-18）在植被沉积物中明显富集（<0.05），而在无植被地点则偏爱（=0.02）。古菌 16S rRNA 基因的定量显示，整个古菌群落、和 Woese-3、Woese-10、Woese-13 和 Woese-21 的绝对丰度在植被沉积物中明显高于裸沉积物（<0.05）。我们的研究扩展了关于古菌在海草系统中的分布模式和生态位偏好的现有知识，特别是对于和的不同亚群，无论是相对比例还是绝对数量。
