Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
Microb Ecol. 2019 Feb;77(2):417-428. doi: 10.1007/s00248-018-1309-7. Epub 2019 Jan 5.
Bathyarchaeota is a diverse, abundant, and widespread archaeal phylum that may play an important role in global carbon cycling. The vertical distribution of Bathyarchaeota and environmental impact on bathyarchaeotal community in deep-sea and lake sediments are known; however, little information is available on Bathyarchaeota in eutrophic and brackish environments, such as mangrove wetlands. In the current study, we investigated the bathyarchaeotal community in the mangrove ecosystem of Futian Nature Reserve, Shenzhen. By slicing the profile into 2-cm layers from the surface to bottom, 110 sediment samples were obtained from three mangrove and three mud flat profiles. High-throughput sequencing of archaeal 16S rRNA genes, quantification of bathyarchaeotal 16S rRNA genes with optimized quantitative primers, and the ensuing statistical analyses revealed the vertical distribution of Bathyarchaeota in the mangrove ecosystem, indicating that Bathyarchaeota was the dominant archaeal phylum therein, with Bathyarchaeota subgroups 6, 8, 15, and 17 as the most abundant subgroups. The abundance of Bathyarchaeota was higher in the mangrove than in the mud flat and other oligotrophic or freshwater habitats. Total organic carbon (TOC) and nitric oxide were significantly correlated with the abundance of Bathyarchaeota, and pH was the major factor shaping the community composition. Further, the data suggested that Bathyarchaeota subgroup 6 preferentially dwelled in slightly acidic, high TOC, and subsurface environments, indicating a potentially distinct role in the global geochemical cycle. These findings expand the knowledge of the distribution and niche preference of Bathyarchaeota, emphasizing the need for continuous characterization of bathyarchaeotal subgroups.
深海底栖古菌是一个多样化、丰富且广泛存在的古菌门，可能在全球碳循环中发挥重要作用。深海底栖古菌在深海和湖底沉积物中的垂直分布及其对底栖古菌群落的环境影响已被人们所了解；然而，在富营养化和半咸水环境中，如红树林湿地，有关底栖古菌的信息却很少。在本研究中，我们调查了深圳福田自然保护区红树林生态系统中的底栖古菌群落。通过从表面到底部将剖面切成 2 厘米厚的层，从三个红树林和三个泥滩剖面中获得了 110 个沉积物样品。通过高通量测序古菌 16S rRNA 基因，使用优化的定量引物定量底栖古菌 16S rRNA 基因，并进行随后的统计分析，揭示了底栖古菌在红树林生态系统中的垂直分布，表明底栖古菌是其中主要的古菌门，底栖古菌亚群 6、8、15 和 17 是最丰富的亚群。底栖古菌的丰度在红树林中高于泥滩和其他贫营养或淡水生境。总有机碳 (TOC) 和一氧化氮与底栖古菌的丰度呈显著正相关，而 pH 是影响群落组成的主要因素。此外，数据表明底栖古菌亚群 6 优先栖息在略酸性、高 TOC 和次表层环境中，这表明其在全球地球化学循环中可能具有独特的作用。这些发现扩展了底栖古菌的分布和生态位偏好的知识，强调需要不断对底栖古菌亚群进行特征描述。
