Wang Yunshu, Liang Honglian, Chen Guoping, Liao Changguang, Wang Yicong, Hu Zongli, Xie Qiaoli
Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing, China.
Front Genet. 2019 Nov 27;10:1222. doi: 10.3389/fgene.2019.01222. eCollection 2019.
The Mediator complex is a multi-subunit protein assembly that serves as a central scaffold to help regulate DNA-binding transcription factors (TFs) and RNA polymerase II (Pol II) activity controlled gene expression programmed in response to developmental or environmental factors. However, litter information about Mediator complex subunit () genes in tomato is available, although it is an essential model plant. In this study, we retrieved 46 candidate genes from the genome of tomato, and a comprehensive analysis was conducted, including their phylogenetic relationship, chromosomal locations, gene structure, cis-regulatory elements prediction, as well as gene expression. The expression profiling of 46 genes was analyzed using publicly available RNA-seq data. Furthermore, we selected some genes to evaluate their expression patterns in various tissues and under different abiotic stress treatments by quantitative reverse transcription PCR experiments. This is the first detailed report to elucidate the molecular and phylogenetic features of the genes in tomato, and it provides valuable clues for further functional analysis in order to clarify the role of the genes in diverse plant growth, development and abiotic stress response.
中介体复合物是一种多亚基蛋白质组装体，作为一个核心支架，帮助调节DNA结合转录因子（TFs）和RNA聚合酶II（Pol II）的活性，从而控制响应发育或环境因素而编程的基因表达。然而，尽管番茄是一种重要的模式植物，但关于番茄中介体复合物亚基（）基因的信息却很少。在本研究中，我们从番茄基因组中检索到46个候选基因，并进行了全面分析，包括它们的系统发育关系、染色体定位、基因结构、顺式调控元件预测以及基因表达。利用公开的RNA-seq数据对46个基因的表达谱进行了分析。此外，我们选择了一些基因，通过定量逆转录PCR实验评估它们在不同组织和不同非生物胁迫处理下的表达模式。这是第一份详细阐述番茄中基因的分子和系统发育特征的报告，为进一步的功能分析提供了有价值的线索，以便阐明基因在植物多种生长、发育和非生物胁迫响应中的作用。
