Department of Laboratory Medicine, Shanghai Children's Medical Centre, Shanghai Jiaotong University School of Medicine, Shanghai, P. R. China.
Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, P. R. China.
Epidemiol Infect. 2019 Dec 13;147:e323. doi: 10.1017/S0950268819002127.
Community-acquired Staphylococcus aureus is a major pathogen responsible for skin and soft tissue infections (SSTIs). This study aimed to investigate the prevalence and molecular characteristics of community-acquired S. aureus isolates recovered from paediatric patients with SSTIs in Shanghai, China. Between January 2015 and January 2018, 91 community-acquired S. aureus isolates were characterised by antibiotic susceptibility, multilocus sequence typing (ST), staphylococcal protein A gene (spa) type and virulence genes. Methicillin-resistant S. aureus (MRSA) strains were also characterised by staphylococcal cassette chromosome mec (SCCmec) type. Forty-one (45.1%) S. aureus isolates were MRSA. ST59 (33.0%, 30/91) was the most common sequence type, and t437 (18.7%, 17/91) was the most common spa type. SCCmec IV and V accounted for 61.0% and 34.1% of all MRSA isolates, respectively. Each isolate carried at least six virulence genes. The positive rates of Panton-Valentine leukocidin genes among all S. aureus, MRSA and methicillin-susceptible S. aureus isolates were 30.8% (28/91), 39.0% (16/41) and 24% (12/50), respectively. The prevalence of community-associated MRSA was surprisingly high among children with community-acquired SSTIs in Shanghai. ST59-t437 was the most prevalent community-acquired S. aureus clone causing SSTIs.
社区获得性金黄色葡萄球菌是引起皮肤和软组织感染（SSTIs）的主要病原体。本研究旨在调查中国上海儿童 SSTIs 患者分离的社区获得性金黄色葡萄球菌的流行情况和分子特征。2015 年 1 月至 2018 年 1 月期间，对 91 株社区获得性金黄色葡萄球菌进行了抗生素敏感性、多位点序列分型（ST）、葡萄球菌蛋白 A 基因（spa）型和毒力基因特征分析。耐甲氧西林金黄色葡萄球菌（MRSA）菌株还通过葡萄球菌盒式染色体 mec（SCCmec）型进行了特征分析。41 株（45.1%）金黄色葡萄球菌为 MRSA。ST59（33.0%，30/91）是最常见的序列型，t437（18.7%，17/91）是最常见的 spa 型。SCCmec IV 和 V 分别占所有 MRSA 分离株的 61.0%和 34.1%。每个分离株至少携带 6 种毒力基因。所有金黄色葡萄球菌、MRSA 和甲氧西林敏感金黄色葡萄球菌分离株中 Panton-Valentine 白细胞毒素基因的阳性率分别为 30.8%（28/91）、39.0%（16/41）和 24%（12/50）。耐甲氧西林社区获得性金黄色葡萄球菌在上海儿童社区获得性 SSTIs 中的流行率令人惊讶地高。ST59-t437 是引起 SSTIs 的最常见社区获得性金黄色葡萄球菌克隆。
