硬骨鱼卵巢组织中类固醇激素代谢的酶

Enzymes of steroid hormone metabolism in ovarian tissue of teleosts.

Eckstein B, Azoury R

Institute of Life Sciences, Hebrew University, Jerusalem, Israel.

Comp Biochem Physiol B. 1979;64(3):309-12. doi: 10.1016/0305-0491(79)90150-0.

Abstract

Substrate-velocity kinetics and some other characteristics of three enzymes involved in steroid hormone metabolism in the ovary of the common carp, Cyprinus carpio and of the grey mullet, Mugil cephalus were determined. 2. Labeled steroid products of the enzyme reactions were identified by thin-layer and gas-liquid chromatographies, as well as by formation of derivatives. 3. 5 alpha-Steroid reductase of carp ovary was found to have a Michaelis-Menten constant undistinguishable from that of rat ovarian tissue, while the Km of the enzyme in mullet ovarian tissue was found to be different. 4. The Km of 20 alpha-hydroxysteroid dehydrogenase of mullet ovarian tissue was similar to the value reported for mammalian gonadal tissue, but the constant for 17 beta-hydroxy-steroid dehydrogenase in the carp ovary was different. 5. It is for the first time that these enzymes of steroid hormone metabolism have been quantitatively determined in teleost ovarian tissue.

摘要

测定了鲤鱼（Cyprinus carpio）和鲻鱼（Mugil cephalus）卵巢中参与类固醇激素代谢的三种酶的底物-速度动力学及其他一些特性。2. 通过薄层色谱法、气液色谱法以及衍生物的形成对酶反应的标记类固醇产物进行了鉴定。3. 发现鲤鱼卵巢的5α-类固醇还原酶的米氏常数与大鼠卵巢组织的米氏常数无法区分，而鲻鱼卵巢组织中该酶的Km值则有所不同。4. 鲻鱼卵巢组织中20α-羟基类固醇脱氢酶的Km值与报道的哺乳动物性腺组织的值相似，但鲤鱼卵巢中17β-羟基类固醇脱氢酶的常数则不同。5. 首次在硬骨鱼卵巢组织中对这些类固醇激素代谢酶进行了定量测定。