Ryan J J, Hattier M M, Adkinson R W, Gough R H
Department of Dairy Sciences, Louisiana Agricultural Experiment Station, Baton Rouge 70803.
J Dairy Sci. 1988 Sep;71(9):2384-7. doi: 10.3168/jds.S0022-0302(88)79822-7.
Effect of disc moistening method on the Bacillus stearothermophilus disc assay zone size was studied. Pasteurized homogenized milk was subdivided and three levels of US Pharmacopeial reference standard penicillin G were added. Disc assay filter paper discs were moistened using the standard capillary action procedure and a method incorporating the use of a 90-microliters micropipetter. Amount of milk sample absorbed by discs prepared by the capillary action method was correlated with disc mass. Variation in amount of milk absorbed by discs was significantly greater when discs were prepared by capillary action. Zone diameter means and standard errors across all penicillin G concentrations tested were not significantly different for the two methods. The 90-microliters micropipetter method is recommended as an alternative to the capillary action method.
研究了圆盘润湿方法对嗜热脂肪芽孢杆菌圆盘测定区大小的影响。将巴氏杀菌均质乳细分，并添加三个水平的美国药典青霉素G参考标准品。圆盘测定滤纸圆盘采用标准毛细作用程序和一种使用90微升微量移液器的方法进行润湿。通过毛细作用法制备的圆盘吸收的牛奶样品量与圆盘质量相关。当通过毛细作用制备圆盘时，圆盘吸收的牛奶量的变化显著更大。对于两种方法，在所有测试的青霉素G浓度下，抑菌圈直径平均值和标准误差没有显著差异。推荐使用90微升微量移液器法作为毛细作用法的替代方法。
